Invitrogen™

InVision™ His-Tag In-Gel Staining Kit

Catalog number:  LC6033

 Related applications:

Protein Electrophoresis & Western Blotting

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Description

The InVision His-tag In-gel Staining Kit includes InVision His-tag In-gel Stain as well as BenchMark His-tagged Protein Standard. InVision His-tag In-gel Stain is a ready-to-use, fast, sensitive, and highly specific stain for visualizing His-tagged fusion protein bands directly in a polyacrylamide gel following electrophoresis.

Features:
• Stain proteins fused to an oligohistidine sequence
• Eliminate western blotting—detect His-tagged proteins directly in the gel
• Detect nanogram levels of protein bands with a UV transilluminator or imaging equipment with appropriate filters or lasers
• Visualize results in under three hours

Compare all His-tag stains ›

Fast and easy staining
The InVision His-tag In-gel Stain protocol is easy to perform and requires very little hands-on-time, allowing for rapid and scalable protein expression screening in a variety of gel types. Following electrophoresis, the gel is fixed, incubated in the stain, washed, and the specific His-tagged fusion protein detected by exposing the gel to UV or visible light. In under three hours, nanogram levels of His-tagged fusion protein can be detected. After documenting your InVision Stain signal, total protein staining can be performed.
WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Range or Limit: nanogram levels
Target Molecule: Proteins (His-Tagged)
Label or Dye: InVision™ His-Tag Stain
Detection Method: Fluorescent
Detection Location: In-Gel Detection
Excitation⁄Emission (nm): 300, 560⁄590
Product Line: InVision™
Product Size: 1 kit

Contents & storage

InVision™ His-tag In-gel Stain is supplied as a 1X ready-to-use staining reagent (500 mL). BenchMark™ His-tagged Protein Standard (125 µL) is also included in the kit.

Documents

Manuals & protocols