InVision™ His-Tag In-Gel Staining Kit

Catalog number: LC6033

Novex™  Related applications: Protein Gel Electrophoresis

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Description

The InVision™ His-tag In-gel Staining Kit includes InVision™ His-tag In-gel Stain (also available separately), as well as BenchMark™ His-tagged Protein Standard. InVision™ His-tag In-gel Stain is a ready-to-use, fast, sensitive, and highly specific stain for visualizing His-tagged fusion protein bands directly in a polyacrylamide gel following electrophoresis. Using InVision™ His-tag In-gel Stain you will:

• Stain proteins fused to an oligohistidine sequence
• Eliminate western blotting-detect His-tagged proteins directly in the gel
• Detect nanogram levels of protein bands with a UV transilluminator, equipped with a standard camera, or a laser-based scanner
• Visualize results in under 3 hours

Convenienty provided as one ready-to-use solution, InVision™ His-tag In-gel Stain does not require mixing, dilution, or solution preparation.

Fast and Easy Staining
The InVision™ His-tag In-gel Stain protocol is easy to perform and requires very little hands-on-time, allowing for rapid and scalable protein expression screening in a variety of gel types. Following electrophoresis, the gel is fixed, incubated in the stain, washed, and the specific His-tagged fusion protein detected by exposing the gel to UV or visible light. In under 3 hours, nanogram levels of His-tagged fusion protein can be detected (Figure 1). After documenting your InVision™ Stain signal, total protein staining can be performed.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Target Molecule: Proteins (His-Tagged)
Label or Dye: InVision™ His-Tag Stain
Detection Method: Fluorescent
Detection Location: In-Gel Detection
Product Size: 1 kit

Contents & storage

InVision™ His-tag In-gel Stain is supplied as a 1X ready-to-use staining reagent (500 mL). BenchMark™ His-tagged Protein Standard (125 µL) is also included in the kit.

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Manuals & protocols

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