Invitrogen™

SilverQuest™ Silver Staining Kit

Catalog number:  LC6070

Related applications:

Protein Electrophoresis & Western Blotting

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Description

The SilverQuest Silver Staining Kit provides a rapid and easy method to silver stain proteins and DNA in polyacrylamide gels. It is specifically designed to provide sensitive silver staining compatible with mass spectrometry analysis.

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Features of this kit:
Compatible—staining compatible with mass spectrometry analysis
Protocol—protein bands (0.3 ng of protein) can be visualized within an hour or for added speed a microwave procedure can be completed in ~30 minutes
Color-coded solutions—color-coded solutions for easy staining protocol

Mass spectrometry-compatible reagents
The SilverQuest Silver Staining Kit has been specifically designed to make protein detection compatible with mass spectrometry analysis. The SilverQuest reagent set contains a sensitizer that contains no glutaraldehyde or formaldehyde, so it leaves lysine and arginine side-chains unmodified. Additionally, the SilverQuest destaining solution effectively removes silver from protein bands in polyacrylamide gels. Peptides remain as efficient targets for in-gel trypsin digestion and gel extraction and yield better coverage during mass spectrometry analysis.
WARNING: Cancer – www.P65Warnings.ca.gov
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Detection Location: In-Gel Detection
Detection Method: Colorimetric
Label or Dye: SilverQuest™ Silver Stain
Product Line: SilverQuest™
Target Molecule: Protein

Contents & storage

This kit contains sufficient reagents to stain 25 minigels

Contains:
250 mL Sensitizer
25 mL Stainer
250 mL Developer
2 mL Developer Enhancer
250 mL Stopper solution
60 mL Destainer A
60 mL Destainer B

Store kit at room temperature (15°C to 30°C). Stable for 6 months.