SilverQuest™ Silver Staining Kit

Catalog number:  LC6070

 Related applications:

Protein Electrophoresis & Western Blotting

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The SilverQuest Silver Staining Kit provides a rapid and easy method to silver stain proteins and DNA in polyacrylamide gels. It is specifically designed to provide sensitive silver staining compatible with mass spectrometry analysis.

Compare all silver stains ›

Features of this kit:
Compatible—staining compatible with mass spectrometry analysis
Protocol—protein bands (0.3 ng of protein) can be visualized within an hour or for added speed a microwave procedure can be completed in ~30 minutes
Color-coded solutions—color-coded solutions for easy staining protocol

Mass spectrometry-compatible reagents
The SilverQuest Silver Staining Kit has been specifically designed to make protein detection compatible with mass spectrometry analysis. The SilverQuest reagent set contains a sensitizer that contains no glutaraldehyde or formaldehyde, so it leaves lysine and arginine side-chains unmodified. Additionally, the SilverQuest destaining solution effectively removes silver from protein bands in polyacrylamide gels. Peptides remain as efficient targets for in-gel trypsin digestion and gel extraction and yield better coverage during mass spectrometry analysis.
WARNING: Cancer –
For Research Use Only. Not for use in diagnostic procedures.


Detection Range or Limit: 0.3ng
Target Molecule: Protein
Label or Dye: SilverQuest™ Silver Stain
Detection Method: Colorimetric
Detection Location: In-Gel Detection
Product Line: SilverQuest™
Product Size: 1 kit

Contents & storage

This kit contains sufficient reagents to stain 25 minigels

250 mL Sensitizer
25 mL Stainer
250 mL Developer
2 mL Developer Enhancer
250 mL Stopper solution
60 mL Destainer A
60 mL Destainer B

Store kit at room temperature (15°C to 30°C). Stable for 6 months.


Manuals & protocols