Kit de tinción con plata SilverQuest™

Citas y referencias (5)

Invitrogen™

Kit de tinción con plata SilverQuest™

El kit de tinción de plata SilverQuest proporciona un método fácil y rápido para la tinción de plata de proteínasMás información

Número de catálogo	Cantidad
LC6070	1 Kit

Número de catálogo LC6070

Precio (MXN)

Cantidad:

1 Kit

Pedido a granel o personalizado

El kit de tinción de plata SilverQuest proporciona un método fácil y rápido para la tinción de plata de proteínas y ADN en geles de poliacrilamida. Está diseñado específicamente para proporcionar una tinción de plata sensible compatible con el análisis de espectrometría de masas.

Compare todas las tinciones de plata ›

Características de este kit:
• Compatible: tinción compatible con análisis de espectrometría de masas
• Protocolo: las bandas de proteínas (0,3 ng de proteína) se pueden visualizar en una hora o, para realizarlo más rápidamente, se puede completar un procedimiento en microondas en ∼ 30 minutos
• Soluciones codificadas por colores: soluciones codificadas por colores para facilitar el protocolo de tinción

Reactivos compatibles con espectrometría de masas
El kit de tinción de plata SilverQuest se ha diseñado específicamente para que la detección de proteínas sea compatible con el análisis de espectrometría de masas. El conjunto de reactivos SilverQuest contiene un sensibilizador que no posee formaldehído o glutaraldehído, por lo que deja las cadenas laterales de lisina y arginina sin modificar. Además, la solución decolorante SilverQuest elimina eficazmente la plata de bandas proteicas en geles de poliacrilamida. Los péptidos permanecen como objetivos eficaces para la digestión de tripsina en gel y la extracción de gel, al tiempo que ofrecen un a mejor cobertura durante el análisis de espectrometría de masas.

Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.

Especificaciones

Ubicación de detecciónDetección en gel

Método de detecciónColorimétrico

Línea de productosSilverQuest

Tipo de productoKit de tinción para geles de proteínas

Cantidad1 Kit

Duración de almacenamiento6 meses

Molécula dianaproteína

Etiqueta o tinteTinción de plata SilverQuest

Unit SizeEach

Contenido y almacenamiento

Este kit contiene suficientes reactivos para teñir 25 minigeles

contiene:
250 ml Sensitizer
25 ml Stainer
250 ml revelador
2 ml revelador ml revelador potenciador
250 ml solución de tope
60 ml Destainer A
60 ml Destainer B

Kit de almacenamiento a temperatura ambiente (15°C a 30°C). Estable 6 durante meses.

Preguntas frecuentes

How much protein is required for mass spectrometry analysis by MALDI-TOF?

In general, the required amount needed to identify a gel-separated protein by enzymatic digestion (usually Trypsin) is about 2 pmole (equivalent to approximately 0.12 µg). Our SilverQuest Silver staining kit is mass spectrometry compatible.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

If you need to stop in the middle of the SilverQuest or SilverXpress silver staining procedure, at what point should this be done?

The sensitizing step is the optimal point to stop the procedure. The gel can be left overnight in the Sensitizing Solution. Although some other kits recommend leaving gels in the fix step, we have found that overnight fixation diminishes stain performances.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is the sensitivity of Coomassie staining and silver staining?

Coomassie sensitivity: 50-500 ng protein per band
Silver-staining sensitivity: 1-5 ng protein per band
In general, silver staining is 10-100 times more sensitive than Coomassie staining.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used the SilverQuest Silver Staining kit and I see negative staining (a dark halo enclosing a pale area). Can you please help me troubleshoot?

This is usually due to overloading of the protein sample. We recommend decreasing the protein load per band. For an unknown protein, a serial dilution may be necessary to determine the best amount to load for a particular protein.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

I used the SilverQuest Silver Staining kit and it took a longer time for band development resulting in a dark background. What happened?

Here are possible causes and solutions:

- Low protein load. Increase the amount of protein load. Be sure to have at least 1-5 ng protein on the gel.
- Some proteins may need longer fixing time. Increase the time for fixing the gel to 2 hours or overnight.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

View all Kit de tinción con plata SilverQuest™ FAQs

Citations & References (5)

Citations & References

Abstract

The Wnt signaling inhibitor dickkopf-1 is required for reentry into the cell cycle of human adult stem cells from bone marrow.

Authors:Gregory CA, Singh H, Perry AS, Prockop DJ,

Journal:J Biol Chem

PubMed ID:12740383

'Adult human mesenchymal stem cells from bone marrow stroma (hMSCs) differentiate into numerous mesenchymal tissue lineages and are attractive candidates for cell and gene therapy. When early passage hMSCs are plated or replated at low density, the cultures display a lag phase of 3-5 days, a phase of rapid exponential ... More

Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon, in neurotransmitter release.

Authors:Takao-Rikitsu E, Mochida S, Inoue E, Deguchi-Tawarada M, Inoue M, Ohtsuka T, Takai Y,

Journal:J Cell Biol

PubMed ID:14734538

'We have recently isolated a novel cytomatrix at the active zone (CAZ)-associated protein, CAST, and found it directly binds another CAZ protein RIM1 and indirectly binds Munc13-1 through RIM1; RIM1 and Munc13-1 directly bind to each other and are implicated in priming of synaptic vesicles. Here, we show that all ... More

The R116C Mutation in alpha A-crystallin Diminishes Its Protective Ability against Stress-induced Lens Epithelial Cell Apoptosis.

Authors: Andley Usha P; Patel Harendra C; Xi Jing-Hua;

Journal:J Biol Chem

PubMed ID:11756414

'alphaA-crystallin is a small heat-shock protein expressed preferentially in the lens and is detected during the early stages of lens development. Recent work indicates that the expression of alphaA-crystallin enhances lens epithelial cell growth and resistance to stress conditions. Mutation of the arginine 116 residue to cysteine (R116C) in alphaA-crystallin ... More

The formation of highly soluble oligomers of alpha-synuclein is regulated by fatty acids and enhanced in Parkinson's disease.

Authors:Sharon R, Bar-Joseph I, Frosch MP, Walsh DM, Hamilton JA, Selkoe DJ,

Journal:Neuron

PubMed ID:12597857

Accumulation of misfolded proteins as insoluble aggregates occurs in several neurodegenerative diseases. In Parkinson's disease (PD) and dementia with Lewy bodies (DLB), alpha-synuclein (alpha S) accumulates in insoluble inclusions. To identify soluble alpha S oligomers that precede insoluble aggregates, we probed the cytosols of mesencephalic neuronal (MES) cells, normal and ... More

The semaphorin receptor plexin-B1 signals through a direct interaction with the Rho-specific nucleotide exchange factor, LARG.

Authors: Aurandt Jennifer; Vikis Haris G; Gutkind J Silvio; Ahn Natalie; Guan Kun-Liang;

Journal:Proc Natl Acad Sci U S A

PubMed ID:12196628

Semaphorins are axon guidance molecules that signal through the plexin family of receptors. Semaphorins also play a role in other processes such as immune regulation and tumorigenesis. However, the molecular signaling mechanisms downstream of plexin receptors have not been elucidated. Semaphorin 4D is the ligand for the plexin-B1 receptor and ... More