Gibco™

Human Melanocyte Basal Medium Kit, calcium free (formerly Medium 254CF Kit)

Catalog number: M254CF500
Have Questions?
Gibco™

Human Melanocyte Basal Medium Kit, calcium free (formerly Medium 254CF Kit)

Catalog number: M254CF500
Have Questions?
Catalog Number
M254CF500
Unit Size
1 kit
Price (USD)
Availability
-
Quantity
Catalog NumberUnit SizePrice (USD)AvailabilityQuantity
M254CF5001 kit
-
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The Gibco Human Melanocyte Basal Medium Kit, Calcium Free (formerly Medium 254CF Kit), enables the culture of human epidermal melanocytes.

The kit contains two components: 
• Human Melanocyte Basal Medium, Calcium Free (formerly Medium 254CF)—a calcium-free version of Human Melanocyte Basal Medium (formerly Medium 254) for those who desire to vary the concentration of calcium 
• A sterile stock solution of calcium chloride (0.2 M, 0.5 mL)
 
Human Melanocyte Basal Medium, Calcium Free, is a basal medium containing essential and non-essential amino acids, vitamins, other organic compounds, trace minerals, and inorganic salts. 
This medium does not contain antibiotics, antimycotics, hormones, growth factors, or proteins. This medium is HEPES and bicarbonate buffered and is designed for use in an incubator with an atmosphere of 5% CO2/95% air. 
 
To support plating and long-term proliferation of normal human melanocytes, Human Melanocyte Basal Medium, Calcium Free, must be supplemented with calcium plus either Human Melanocyte Growth Supplement (HMGS, Cat. No. S-002-5) or PMA-Free Human Melanocyte Growth Supplement-2 (HMGS-2, Cat. No. S-016-5). Each of these supplements contains all of the growth factors, hormones, and tissue extracts necessary for growth of melanocytes in Human Melanocyte Basal Medium, Calcium Free.
 
Note: Calcium chloride must be added to this medium prior to use. Calcium concentration from other sources is 0.5 μM in unsupplemented Human Melanocyte Basal Medium, Calcium Free.

 

For Research Use Only. Not for use in diagnostic procedures.

Specifications

Cell Type
Melanocytes
Culture Type
Mammalian Cell Culture
Form
Liquid
Product Type
Medium 254CF Kit
Sterility
Sterile-filtered
Sterilization Method
Sterile-filtered
With Additives
Phenol Red
Without Additives
No Calcium Chloride
Green Features
Sustainable packaging
Product Line
Cascade Biologics™
Quantity
1 kit
Shipping Condition
Wet ice

Contents & Storage

  • 500 mL Human Melanocyte Basal Medium, Calcium Free (formerly “Medium 254CF”); store in refrigerator (2–8°C) and protect from light
  • Sterile stock solution of calcium chloride (0.2 M, 0.5 mL); store in refrigerator (2–8°C)

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