La sal de potasio Magnesium Green™, con permeabilidad celular muestra una afinidad superior para Mg2+ (Kd ∼1,0 mM) que mag-fura-2Más información
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Número de catálogo
Cantidad
M3733
1 mg
Número de catálogo M3733
Precio (MXN)
-
Cantidad:
1 mg
La sal de potasio Magnesium Green™, con permeabilidad celular muestra una afinidad superior para Mg2+ (Kd ∼1,0 mM) que mag-fura-2 (Kd ∼1,9 mM) o mag-indo-1 (Kd ∼2,7 mM). Al unir Mg2+, Magnesium Green muestr y aumenta la intensidad de emisión de fluorescencia sin cambiar la longitud de onda con una absorción/emisión máxima de ∼506/531 nm.
Para uso exclusivo en investigación. No apto para uso en procedimientos diagnósticos.
Especificaciones
Método de detecciónFluorescente
Tipo de coloranteIndicador de magnesio
Cantidad1 mg
Condiciones de envíoTemperatura ambiente
Para utilizar con (equipo)Microscopio de fluorescencia, Lector de microplacas
Línea de productosVerde magnesio
Tipo de productoSal de pentampotasio
Unit SizeEach
Contenido y almacenamiento
Almacenar en el congelador de -5 °C a -30 °C y proteger de la luz.
Citations & References (27)
Citations & References
Abstract
Presynaptic Ca(2+) influx at the inhibitor of the crayfish neuromuscular junction: a photometric study at a high time resolution.
Authors:Vyshedskiy A, Lin JW
Journal:J Neurophysiol
PubMed ID:10634895
'Presynaptic calcium influx at the inhibitor of the crayfish neuromuscular junction was investigated by measuring fluorescence transients generated by calcium-sensitive dyes. This approach allowed us to correlate presynaptic calcium influx with transmitter release at a high time resolution. Systematic testing of the calcium indicators showed that only low-affinity dyes, with ... More
Requirement for an additional divalent metal cation to activate protein tyrosine kinases.
Authors:Sun G, Budde RJ
Journal:Biochemistry
PubMed ID:9047313
'In addition to the magnesium ion needed to form the true phosphate-donating substrate (ATP-Mg complex), we have determined that at least one additional Mg2+ ion is essential for the activation of protein tyrosine kinases. This activation was investigated in detail using purified Csk, Src, and the fibroblast growth factor receptor ... More
Ca2+ fluorescence imaging with pico- and femtosecond two-photon excitation: signal and photodamage.
Authors:Koester HJ, Baur D, Uhl R, Hell SW
Journal:Biophys J
PubMed ID:10512842
'The signal and limitations of calcium florescence imaging using nonresonant multiphoton absorption of near-infrared femto- and picosecond laser pulses were examined. The fluorescence changes of various Ca(2+)-indicators induced by transient increases of the intradendritic calcium concentration were evaluated by evoking physiological activity in neocortical neurons in rat brain slices. Photodamage ... More
Effects of magnesium on cyclic GMP hydrolysis by the bovine retinal rod cyclic GMP phosphodiesterase.
Authors:Srivastava D, Fox DA, Hurwitz RL
Journal:Biochem J
PubMed ID:7772055
'Knowledge of the kinetics of the rod cyclic GMP phosphodiesterase is essential for understanding the kinetics and gain of the light response. Therefore, the interactions between Mg2+, cyclic GMP, and purified, trypsin-activated bovine rod cyclic GMP phosphodiesterase (EC 3.1.4.17) were examined. The effects of Mg2+ and of cyclic GMP on ... More
Mechanisms of calcium influx into hippocampal spines: heterogeneity among spines, coincidence detection by NMDA receptors, and optical quantal analysis.
Authors:Yuste R, Majewska A, Cash SS, Denk W
Journal:J Neurosci
PubMed ID:10066251
'Dendritic spines receive most excitatory inputs in the vertebrate brain, but their function is still poorly understood. Using two-photon calcium imaging of CA1 pyramidal neurons in rat hippocampal slices, we investigated the mechanisms by which calcium enters into individual spines in the stratum radiatum. We find three different pathways for ... More