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Citations & References (139)
Invitrogen™
SPQ (6-Methoxy-N-(3-Sulfopropyl)Quinolinium, Inner Salt)
The chloride indicator SPQ has absorption/emission maxima ∼344/443 and detects Cl- via diffusion-limited collisional quenching.Learn more about ion indicators includingRead more
| Catalog Number | Quantity |
|---|---|
| M440 | 100 mg |
Catalog number M440
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Quantity:
100 mg
The chloride indicator SPQ has absorption/emission maxima ∼344/443 and detects Cl- via diffusion-limited collisional quenching.
Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›
Learn more about ion indicators including calcium, potassium, pH, and membrane potential indicators ›
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection MethodFluorescence
Dye TypeFluorescent Dye-Based
Quantity100 mg
Shipping ConditionRoom Temperature
For Use With (Equipment)Confocal Microscope, Flow Cytometer
Product TypeChloride Indicator
Unit SizeEach
Contents & Storage
Store at room temperature and protect from light.
Citations & References (139)
Citations & References
Abstract
Identification by site-directed mutagenesis of three arginines in uncoupling protein that are essential for nucleotide binding and inhibition.
Journal:J Biol Chem
PubMed ID:9312070
Primary regulation of uncoupling protein is mediated by purine nucleotides, which bind to the protein and allosterically inhibit fatty acid-induced proton transport. To gain increased understanding of nucleotide regulation, we evaluated the role of basic amino acid residues using site-directed mutagenesis. Mutant and wild-type proteins were expressed in yeast, purified,
Fluorescent indicators of ion concentrations.
Journal:Methods Cell Biol
PubMed ID:2538708
Efficient endocytosis of the cystic fibrosis transmembrane conductance regulator requires a tyrosine-based signal.
Journal:J Biol Chem
PubMed ID:9920908
'We previously demonstrated that the cystic fibrosis transmembrane conductance regulator (CFTR) is rapidly endocytosed in epithelial cells (Prince, L. S., Workman, R. B., Jr., and Marchase, R. B. (1994) Proc. Natl. Acad. Sci. U. S. A. 91, 5192-5196). To determine the structural features of CFTR required for endocytosis, we prepared
Estimation of intracellular chloride activity in isolated perfused rabbit proximal convoluted tubules using a fluorescent indicator.
Journal:Biophys J
PubMed ID:3395662
'The methodology has been developed to measure cell chloride activity by fluorescence microscopy using the chloride-sensitive dye, 6-methoxy-1-(3-sulfonatopropyl)quinolinium (SPQ). SPQ was loaded into cells of the in vitro microperfused rabbit proximal convoluted tubule by a 10 min luminal perfusion with 20 mM SPQ at 38 degrees C. Fluorescence was excited
Regulation of Cl/HCO3 exchange in gastric parietal cells.
Journal:Cell Regul
PubMed ID:1742343
'Microspectrofluorimetry of the fluorescent indicators 2',7'-bis-(2-carboxyethyl)-5(and-6)carboxyfluorescein and 6-methoxy-N-(3-sulfopropyl)-quinolinium was used to measure intracellular pH (pHi), intracellular Cl (Cli), and transmembrane fluxes of HCO3 and Cl in single parietal cells (PC) in isolated rabbit gastric glands incubated in HCO3/CO2-buffered solutions. Steady-state pHi was 7.2 in both resting (50 microM cimetidine) and