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Invitrogen
DIABLO Monoclonal Antibody (SMAC 17 1-87)
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FIGURE: 1 / 11
DIABLO Antibody (MA1-936) in ICC/IF
Immunofluorescence analysis of DIABLO was performed using log phase HeLa cells treated with 25uM of Etoposide for 1 hour. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with DIABLO Mouse Monoclonal Antibody (Product # MA1-936) at 5 µg/mL in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Pr... View More
Product Details
MA1-936
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Published species
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
SMAC 17 1-87
Immunogen
C-Terminal His tagged recombinant human Smac/DIABLO.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-936 detects Smac/DIABLO from human, mouse and rat tissues.
MA1-936 has been successfully used in Immunoprecipitation, Western blot, Immunohistochemistry (paraffin), Immunofluorescence and immunocytochemistry. By Western blot it detects a 25-kDa band representing Smac/DIABLO.
MA1-936 antigen is C-term His tagged recombinant human Smac/DIABLO.
Target Information
DIABLO (smac), a mitochondrial protein, activates various forms of apoptosis. This activation may be due to the neutralization of one or more members of the IAP family of apoptosis inhibitory proteins. Smac exits the mitochondria and enter the cytosol during certain apoptosis triggered events. Mitochondrial-mediated apoptosis is important in animal development and tissue homeostasis, with alterations resulting in a range of malignant disorders. Upon apoptotic stimuli, the mitochondrial proteins cytochrome c and Smac/DIABLO are released into the cytosol. The release of these proteins, however, occurs via different mechanisms. Smac/DIABLO has also been found to play a key role in regulating the sensitization of cancer cells to apoptosis (both immune and drug-induced).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: antagonist of IAPs (inhibitors of apoptosis); diablo homolog (Drosophila) isoform DIABLO_1; diablo homolog (Drosophila) isoform DIABLO_2; Diablo homolog, mitochondrial; Diablo IAP-binding mitochondrial protein; diablo-like protein; DIABLO-S; direct IAP binding protein with low PI; Direct IAP-binding protein with low pI; FLJ10537; FLJ25049; hCG_1782202; Second mitochondria-derived activator of caspase; Second mitochondria-derived activator of caspases; short isoform; SMAC; Smac protein; SMAC3; unnamed protein product
Gene Aliases: 0610041G12Rik; 1700006L01Rik; AU040403; DFNA64; DIABLO; SMAC
UniProt ID: (Human) Q9NR28, (Mouse) Q9JIQ3
Entrez Gene ID: (Human) 56616, (Mouse) 66593, (Rat) 288753
apoptotic process
activation of cysteine-type endopeptidase activity involved in apoptotic process
intrinsic apoptotic signaling pathway in response to oxidative stress
activation of cysteine-type endopeptidase activity involved in apoptotic process by cytochrome c
positive regulation of apoptotic process
neuron apoptotic process
intrinsic apoptotic signaling pathway
extrinsic apoptotic signaling pathway via death domain receptors
apoptotic signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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