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          • Primary Antibodies ›
          • TRA-1-60 Antibodies

          Invitrogen

          TRA-1-60 Monoclonal Antibody (TRA-1-60)

          2 Published Figures
          18 References
          View all (10) TRA-1-60 antibodies

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          Cite TRA-1-60 Monoclonal Antibody (TRA-1-60)

          Additional Information:
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          • Antibody Testing Data (7)
          • Published Figures (2)
          TRA-1-60 Antibody in Immunocytochemistry (ICC/IF)
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          TRA-1-60 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 9

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          TRA-1-60 Antibody (MA1-023) in ICC/IF

          Immunofluorescent analysis of TRA-1-60 using anti-TRA-1-60 monoclonal antibody (Product # MA1-023) shows staining on the cell surface of human H9 human embryonic stem cells, indicating pluripotency. TRA-1-60 staining (green) and an overlay image of TRA-1-60 with DAPI (blue) is shown. H9 human embryonic stem cells were grown on matrigel coated chamber slides and fixed with formaldehyde prior to staining. Cells were probed with a Monoclonal antibody recognizing TRA-1-60 (Product # MA1-023) at a dilution of 1:20 over night at 4 °C, washed wit... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          View Product

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          TRA-1-60 Antibody in Immunocytochemistry (ICC/IF)
          TRA-1-60 Antibody in Immunocytochemistry (ICC/IF)
          TRA-1-60 Antibody in Immunocytochemistry (ICC/IF)
          TRA-1-60 Antibody in Western Blot (WB)
          TRA-1-60 Antibody in Flow Cytometry (Flow)
          TRA-1-60 Antibody in Flow Cytometry (Flow)
          TRA-1-60 Antibody in Flow Cytometry (Flow)
          TRA-1-60 Antibody in Immunocytochemistry (ICC/IF)
          TRA-1-60 Antibody in Immunohistochemistry, Immunocytochemistry (IHC, ICC/IF)

          Product Details

          MA1-023

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:500-1:2,000
          -

          Immunohistochemistry (IHC)

          -
          View 1 publication 1 publication

          Immunocytochemistry (ICC/IF)

          1:20-1:100
          View 14 publications 14 publications

          Flow Cytometry (Flow)

          0.4 µg
          View 1 publication 1 publication

          Miscellaneous PubMed (Misc)

          -
          View 2 publications 2 publications
          Product Specifications

          Species Reactivity

          Human

          Published species

          Bovine, Human, Mouse

          Host/Isotype

          Mouse / IgM

          Class

          Monoclonal

          Type

          Antibody

          Clone

          TRA-1-60

          Immunogen

          Human embryonal carcinoma cell line 2102Ep.

          Conjugate

          Unconjugated Unconjugated Unconjugated
          • DyLight 488
          • DyLight 550
          • DyLight 650
          • PE 
          • Request custom conjugation

          Form

          Liquid

          Concentration

          1 mg/mL

          Purification

          Affinity chromatography - MBP

          Storage buffer

          PBS, pH 7.4, with 1mg/mL BSA

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2536699

          Target Information

          TRA-1-60 is a cell surface antigen, expressed along with SSEA-3, SSEA-4 and TRA-1-81 in human embryonic stem cells, embryonal carcinoma cells and induced pluripotent stem cells (iPS). These surface markers are lost during the differentiation process. In contrast, SSEA-1 is absent in undifferentiated human stem cells but is present on the cell surface after retinoic acid mediated differentiation.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          It has to be done as per old AB suggested Products section.

          Disclaimer

          Close

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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