Search
Invitrogen
CX3CL1 Monoclonal Antibody (4C5)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 4
CX3CL1 Antibody (MA1-031) in WB
Western blot was performed using Anti-CX3CL1 Monoclonal Antibody (4C5) (Product # MA1-031) and a 85 kDa band corresponding to soluble cleaved fragment of CX3CL1 was observed across tested cell line. Conditioned media (20 µg µg lysate) of HUVEC (Lane 1), HUVEC treated with TNF-alpha, 10 ng/mL for 12 hrs (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-031
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG
Class
Monoclonal
Type
Antibody
Clone
4C5
Immunogen
Non-glycosylated, polypeptide chain containing 76 amino acids of chemokine domain expressed in E. coli.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA, 50% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target Information
Chemokines are a family of proteins associated with the trafficking of leukocytes in immune surveillance and inflammatory cell recruitment. They are classified based on the positions of key cysteine residues. CX3CL1 is a CX3C chemokine known to induce adhesion and migration of leukocytes mediated by a membrane-bound and soluble form respectively. Recent experiments have shown that CX3CL1 can suppress the production of nitrous oxide, interleukin-6, and TNF-a in activated microglia and neuronal cells, suggesting that it may act as an intrinsic inhibitor against neurotoxicity by activated microglia. Its receptor, CX3CR1, also functions as a co-receptor for HIV-1 and HIV-2 envelope fusion and virus infection, which can be inhibited by CX3CL1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: C-X3-C motif chemokine 1; chemokine (C-X3-C motif) ligand 1; CX3C chemokine; CX3C membrane-anchored chemokine; CX3CL1; neurotactin; small inducible cytokine subfamily D (Cys-X3-Cys), member 1 (fractalkine, neurotactin); small inducible cytokine subfamily D (Cys-X3-Cys), member-1; small inducible cytokine subfamily D, 1; small-inducible cytokine D1; unnamed protein product
Gene Aliases: ABCD-3; C3Xkine; CX3C; CXC3; CXC3C; D8Bwg0439e; FK; fractalkine; neurotactin; NTN; NTT; SCYD1
UniProt ID: (Human) P78423, (Mouse) O35188
Entrez Gene ID: (Human) 6376, (Mouse) 20312
microglial cell activation
positive regulation of neuroblast proliferation
leukocyte migration involved in inflammatory response
response to ischemia
chemotaxis
inflammatory response
immune response
cell adhesion
G-protein coupled receptor signaling pathway
positive regulation of cell proliferation
positive regulation of macrophage chemotaxis
positive regulation of neuron projection development
cell migration
cytokine-mediated signaling pathway
positive regulation of cell migration
negative regulation of cell migration
neutrophil chemotaxis
regulation of lipopolysaccharide-mediated signaling pathway
positive regulation of actin filament bundle assembly
negative regulation of interleukin-1 alpha production
negative regulation of tumor necrosis factor production
positive regulation of transforming growth factor beta1 production
integrin activation
autocrine signaling
wound healing
negative regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of MAPK cascade
positive regulation of angiogenesis
positive regulation of cell adhesion
negative regulation of vasoconstriction
positive regulation of transcription from RNA polymerase II promoter
eosinophil chemotaxis
macrophage chemotaxis
lymphocyte chemotaxis
positive regulation of smooth muscle cell proliferation
positive regulation of inflammatory response
positive chemotaxis
positive regulation of calcium-independent cell-cell adhesion
positive regulation of release of sequestered calcium ion into cytosol
positive regulation of protein kinase B signaling
angiogenesis involved in wound healing
inhibitory postsynaptic potential
cell chemotaxis
microglial cell proliferation
antimicrobial humoral immune response mediated by antimicrobial peptide
neuron cellular homeostasis
chemokine-mediated signaling pathway
cellular response to tumor necrosis factor
cell-cell adhesion
negative regulation of hippocampal neuron apoptotic process
negative regulation of glutamate receptor signaling pathway
negative regulation of microglial cell activation
positive regulation of microglial cell migration
negative regulation of apoptotic signaling pathway
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
positive regulation of cell-matrix adhesion
defense response
cell-cell signaling
negative regulation of cell-substrate adhesion
neuron remodeling
leukocyte chemotaxis
negative regulation of interleukin-1 beta production
negative regulation of interleukin-6 production
regulation of synaptic plasticity
regulation of neurogenesis
leukocyte adhesive activation
positive regulation of ERK1 and ERK2 cascade
synapse pruning
negative regulation of neuron migration
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support