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Invitrogen
Adiponectin Monoclonal Antibody (19F1)
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FIGURE: 1 / 34
Adiponectin Antibody (MA1-054) in ICC/IF
Immunofluorescence analysis of Adiponectin was performed using 70% confluent 3T3-L1 cells differentiated with StemPro Adipogenesis Supplement (Product # A10070-01) for 5 days. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Adiponectin (19F1) Mouse Monoclona... View More
Product Details
MA1-054
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rabbit
Published species
Bovine,
Human,
Mouse,
Rabbit
Host/Isotype
Mouse
/ IgG
Class
Monoclonal
Type
Antibody
Clone
19F1
Immunogen
Full length, recombinant, human adiponectin.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-054 detects adiponectin from human, rabbit and mouse samples.
MA1-054 has been used successfully in Western blot, immunofluorescence, and ELISA procedures. By Western blot this antibody detects ~30 kDa protein representing Adiponectin in 3T3-L1 adipocytes. Immunofluorescence and ELISA of Adiponectin can also be performed using MA1-054.
The MA1-054 immunogen is full length, recombinant, human adiponectin.
Target Information
Adiponectin is a protein hormone secreted exclusively by adipocytes that regulates the metabolism of lipids and glucose. Adiponectin has been shown to play a role in various physiological processes including homeostasis and obesity. Decreased levels of adiponectin are associated with obese individuals as well as insulin resistance and hyperinsulinemia (type 2 diabetes). In mice adiponectin has been shown to improve insulin signaling, thus ameliorating insulin resistance. Through activation of AMP kinase and PPAR-alpha, adiponectin can increase fatty acid transport, oxidation, and dissipation in skeletal muscle, leading to a reduction of intramyocellular lipid levels and thus improved insulin signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 30 kDa adipocyte complement-related protein; ACRP30; adipocyte complement related protein; Adipocyte complement-related 30 kDa protein; adipocyte complement-related protein of 30 kDa; adipocyte, C1Q and collagen domain containing; Adipocyte, C1q and collagen domain-containing protein; Adipocyte-specific protein AdipoQ; Adiponectin; Adipose most abundant gene transcript 1 protein; adipose specific collagen-like factor; ADPIOQ; apM-1; Gelatin-binding protein; gelatin-binding protein 28; unnamed protein product
Gene Aliases: 30kDa; ACDC; ACRP30; Ad; adipo; ADIPOQ; ADIPQTL1; ADPN; APM-1; APM1; APN; GBP28
UniProt ID: (Human) Q15848, (Mouse) Q60994
Entrez Gene ID: (Rabbit) 100009027, (Human) 9370, (Mouse) 11450
response to hypoxia
positive regulation of protein phosphorylation
glucose metabolic process
generation of precursor metabolites and energy
fatty acid beta-oxidation
response to nutrient
circadian rhythm
response to xenobiotic stimulus
response to bacterium
response to sucrose
response to glucose
positive regulation of signal transduction
gene expression
negative regulation of platelet-derived growth factor receptor signaling pathway
negative regulation of macrophage derived foam cell differentiation
negative regulation of tumor necrosis factor-mediated signaling pathway
positive regulation of cholesterol efflux
regulation of glucose metabolic process
response to activity
fatty acid oxidation
negative regulation of cell migration
negative regulation of granulocyte differentiation
response to nutrient levels
negative regulation of protein autophosphorylation
negative regulation of tumor necrosis factor production
positive regulation of interleukin-8 production
cellular response to insulin stimulus
positive regulation of myeloid cell apoptotic process
adiponectin-activated signaling pathway
negative regulation of heterotypic cell-cell adhesion
low-density lipoprotein particle clearance
response to tumor necrosis factor
glucose homeostasis
positive regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of I-kappaB kinase/NF-kappaB signaling
negative regulation of MAP kinase activity
response to ethanol
negative regulation of fat cell differentiation
negative regulation of macrophage differentiation
negative regulation of gluconeogenesis
negative regulation of blood pressure
negative regulation of transcription, DNA-templated
positive regulation of fatty acid metabolic process
positive regulation of glucose import
negative regulation of hormone secretion
negative regulation of receptor-mediated endocytosis
negative regulation of inflammatory response
negative regulation of phagocytosis
negative regulation of synaptic transmission
brown fat cell differentiation
response to glucocorticoid
negative regulation of cholesterol import
negative regulation of ERK1 and ERK2 cascade
response to linoleic acid
detection of oxidative stress
cellular response to cAMP
cellular response to xenobiotic stimulus
positive regulation of monocyte chemotactic protein-1 production
cellular response to epinephrine stimulus
protein localization to plasma membrane
negative regulation of intracellular protein transport
positive regulation of cold-induced thermogenesis
negative regulation of cold-induced thermogenesis
positive regulation of cAMP/PKA signal transduction
negative regulation of receptor binding
negative regulation of vascular smooth muscle cell proliferation
negative regulation of vascular associated smooth muscle cell migration
negative regulation of DNA biosynthetic process
positive regulation of glycogen (starch) synthase activity
positive regulation of metanephric glomerular visceral epithelial cell development
positive regulation of cAMP-dependent protein kinase activity
positive regulation of renal albumin absorption
negative regulation of platelet-derived growth factor receptor-alpha signaling pathway
negative regulation of metanephric mesenchymal cell migration
positive regulation of protein kinase A signaling
negative regulation of smooth muscle cell migration
positive regulation of cellular protein metabolic process
cellular response to drug
negative regulation of low-density lipoprotein particle receptor biosynthetic process
positive regulation of blood pressure
positive regulation of protein kinase activity
negative regulation of smooth muscle cell proliferation
positive regulation of peptidyl-tyrosine phosphorylation
protein homooligomerization
membrane depolarization
membrane hyperpolarization
protein heterotrimerization
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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