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Invitrogen
Cytokeratin 19 Monoclonal Antibody (RCK108)
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FIGURE: 1 / 5
Cytokeratin 19 Antibody (MA1-06329) in ICC/IF
Immunofluorescence analysis of Cytokeratin 19 was performed using 70% confluent log phase MCF7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Cytokeratin 19 Mouse Monoclonal Antibody (RCK108) (Product # MA1-06329) at 5 µg/mL in 0.1% BSA, incubated at... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-06329
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Zebrafish
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
RCK108
Immunogen
Cytoskeletal preparation of human bladder carcinoma cell line T24.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
purified
Storage buffer
PBS
Contains
0.09% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-06329 detects cytokeratin 19 in human and zebrafish samples.
MA1-06329 has sucessfully been used in Western blotting, flow cytometry, immunocytochemistry and immunohistochemistry.
The MA1-06329 immunogen is a cytoskeletal preparation of human bladder carcinoma cell line T24.
Target Information
Cytokeratin 19 is part of a subfamily of intermediate filament proteins and are characterized by a remarkable biochemical diversity, represented in human epithelial tissues by at least 20 different polypeptides. Cytokeratins range in molecular weight from 40-68 kDa and isoelectric pH between 4.9 - 7.8. The individual human cytokeratins are numbered 1 to 20. The various epithelia in the human body usually express cytokeratins which are not only characteristic of the type of epithelium, but also related to the degree of maturation or differentiation within an epithelium. Cytokeratin subtype expression patterns are used to an increasing extent in the distinction of different types of epithelial malignancies. The cytokeratin antibodies are not only of assistance in the differential diagnosis of tumors using immunohistochemistry on tissue sections, but are also a useful tool in cytopathology and flow cytometric assays. For example, cytokeratin 19 is not expressed in hepatocytes, therefore, it is useful in the identification of liver metastasis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 40-kDa keratin intermediate filament; C K1CS; CK 19; CK-19; cytokeratin 19; keratin 19, type I; keratin, type I, 40-kd; MGC15366; unnamed protein product
Gene Aliases: CK19; K19; K1CS
UniProt ID: (Human) P08727
Entrez Gene ID: (Human) 3880
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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