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Invitrogen
NUP62 Monoclonal Antibody (RL31)
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FIGURE: 1 / 1
NUP62 Antibody (MA1-073) in WB
Western blot analysis of Nucleoporin p62 was performed by loading whole cell lysates (20 µg) of the indicated samples and 5 µL of Thermo Scientific PageRuler Plus Prestained Protein Ladder (Product # 26619) onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Fast Blotter (Product # 62288), and blocked with 5% milk in TBST for 1 hour at room temperature. Nucleoporin p62 was detected at ~62 kD in samples using a Nucleoporin p62 (RL31) monoclonal antibody (Product # MA1-073) at a d... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-073
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Armenian hamster
/ IgG
Class
Monoclonal
Type
Antibody
Clone
RL31
Immunogen
O-linked glycoprotein-enriched fraction from rat liver nuclear envelopes raised in Armenian hamsters.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2-7.4, with 30% glycerol, 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-073 detects the nucleoporin p62 O-linked glycoprotein in the p62 complex from rat, human and non-human primate tissues.
MA1-073 has been successfully used in Western blot and immunofluorescence procedures. By Western blot, this antibody detects a single ~62 kDa protein representing nucleoporin p62 in rat liver nuclear envelope fractions. Immunofluorescence staining of p62 in rat liver with MA1-073 results in staining of both the nucleoplasmic and cytoplasmic surfaces of the nuclear pore complex (NPC).
The MA1-073 immunogen is O-linked glycoprotein-enriched fraction from rat liver nuclear envelopes raised in Armenian hamsters.
Target Information
SDS3 is a subunit of the histone deacetylase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 62 kDa nucleoporin; DKFZp547L134; FLJ20822; FLJ43869; MGC841; nuclear pore complex 1; nuclear pore glycoprotein 62; nucleoporin 62kD; nucleoporin 62kDa; nucleoporin Nup62; nucleoporin p62; nucleoporin p62 homolog; NUP62; unnamed protein product
Gene Aliases: D7Ertd649e; IBSN; Np62; Nupc1; p62; SNDI
UniProt ID: (Human) P37198, (Mouse) Q63850, (Rat) P17955
Entrez Gene ID: (Dog) 484370, (Human) 23636, (Mouse) 18226, (Rat) 65274
chromatin binding
protein binding
phospholipid binding
structural constituent of nuclear pore
receptor signaling complex scaffold activity
Hsp70 protein binding
SH2 domain binding
ubiquitin binding
PTB domain binding
Hsp90 protein binding
kinesin binding
macromolecular complex binding
thyroid hormone receptor binding
transporter
RNA export from nucleus
protein import into nucleus
nucleocytoplasmic transport
mitotic metaphase plate congression
centrosome cycle
mitotic centrosome separation
cell surface receptor signaling pathway
negative regulation of cell proliferation
regulation of signal transduction
negative regulation of epidermal growth factor receptor signaling pathway
negative regulation of apoptotic process
negative regulation of programmed cell death
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of epidermal growth factor receptor signaling pathway
positive regulation of mitotic nuclear division
positive regulation of transcription, DNA-templated
regulation of Ras protein signal transduction
negative regulation of Ras protein signal transduction
positive regulation of centriole replication
regulation of mitotic spindle organization
centriole assembly
positive regulation of mitotic cytokinetic process
positive regulation of protein localization to centrosome
cell migration
regulation of protein import into nucleus
negative regulation of MAP kinase activity
cellular senescence
spermatogenesis
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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