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FIGURE: 1 / 8
SHIP1 Antibody (MA1-10449) in ICC/IF
Immunofluorescence analysis of SHIP1 was performed using 70% confluent log phase THP-1 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with SHIP1 Mouse Monoclonal Antibody (SHIP-01) (Product # MA1-10449) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A3... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-10449
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
SHIP-01
Immunogen
Peptide coresponding to a sequence within N-terminal domain of Human SHIP-1.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with SHIP-1, a phosphoinositide phosphatase largely confined to hematopoietic cells (intracellular antigen). Multiple forms of SHIP-1 have been reported with molecular weights of 110, 125, 130, 135 and 145 kDa.
Western Blot: Reducing conditions.
Target Information
This gene is a member of the inositol polyphosphate-5-phosphatase (INPP5) family and encodes a protein with an N-terminal SH2 domain, an inositol phosphatase domain, and two C-terminal protein interaction domains. Expression of this protein is restricted to hematopoietic cells where its movement from the cytosol to the plasma membrane is mediated by tyrosine phosphorylation. At the plasma membrane, the protein hydrolyzes the 5' phosphate from phosphatidylinositol (3,4,5)-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, thereby affecting multiple signaling pathways. Overall, the protein functions as a negative regulator of myeliod cell proliferation and survival. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: inositol polyphosphate-5-phosphatase, 145kD; inositol polyphosphate-5-phosphatase, 145kDa; MGC104855; MGC142140; MGC142142; OTTHUMP00000165069; OTTHUMP00000165070; OTTHUMP00000165071; OTTHUMP00000165072; OTTHUMP00000203442; phosphatidylin; phosphatidylinositol 4,5-bisphosphate 5-phosphatase; SH2 domain-containing inositol 5'-phosphatase 1; signaling inositol polyphosphate 5 phosphatase SIP-145; signaling inositol polyphosphate phosphatase SHIP II; unnamed protein product
Gene Aliases: hp51CN; p150Ship; SHIP; SHIP-1; SHIP1; SIP-145
UniProt ID: (Human) Q92835
Entrez Gene ID: (Human) 3635
phosphatidylinositol-4,5-bisphosphate 5-phosphatase activity
inositol-polyphosphate 5-phosphatase activity
protein binding
phosphatidylinositol-3,4,5-trisphosphate 3-phosphatase activity
phosphatase activity
phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase activity
inositol-1,4,5-trisphosphate 5-phosphatase activity
inositol-1,3,4,5-tetrakisphosphate 5-phosphatase activity
phosphatidylinositol biosynthetic process
phosphate-containing compound metabolic process
signal transduction
negative regulation of signal transduction
positive regulation of B cell differentiation
negative regulation of neutrophil differentiation
negative regulation of bone resorption
negative regulation of natural killer cell mediated cytotoxicity
phosphatidylinositol dephosphorylation
regulation of immune response
T cell receptor signaling pathway
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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