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Invitrogen

PAX8 Monoclonal Antibody (1F8-3A8)

Advanced Verification
3 Published Figures
7 References
View all (83) PAX8 antibodies

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Product Details

MA1-117

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
-

Immunohistochemistry (IHC)

-
View 2 publications 2 publications

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:200
-

Immunohistochemistry (Frozen) (IHC (F))

-
View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

1:50-1:200
View 2 publications 2 publications

Flow Cytometry (Flow)

-
View 2 publications 2 publications
Product Specifications

Species Reactivity

Human, Mouse, Non-human primate

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

1F8-3A8

Immunogen

Purified internal fragment of human recombinant PAX8 expressed in E. coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Amount

100 µg

Purification

Protein A

Storage buffer

PBS with 1mg/mL BSA, 30% glycerol

Contains

0.05% sodium azide

Storage conditions

-20°C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2536828

Product Specific Information

Western blot analysis of MA1-117 detects a prominent ~48 kDa protein in human adenocarcinoma SKOV-3 and SV40 transformed African green monkey (Cercopithecus aethiops) kidney cells. In SKOV-3 cells, additional unknown low molecular bands are also detected. Specificity of the antibody was confirmed in HeLa (negative control) and 293T cells overexpressing full length PAX8.

Target Information

This gene encodes a member of the paired box family of transcription factors. Members of this gene family typically encode proteins that contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes. Mutations in this gene have been associated with thyroid dysgenesis, thyroid follicular carcinomas and atypical follicular thyroid adenomas. Alternatively spliced transcript variants encoding different isoforms have been described.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: HGNC:8622; paired box gene 8; paired domain gene 8; paired-box-protein; Pax8; PAX8d; PAX8e; PAX8F; unnamed protein product

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Gene Aliases: PAX-8

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UniProt ID: (Human) Q06710, (Mouse) Q00288

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Entrez Gene ID: (Human) 7849, (Mouse) 18510

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Function(s)
transcription regulatory region sequence-specific DNA binding RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II transcription factor activity, sequence-specific DNA binding DNA binding transcription factor activity, sequence-specific DNA binding thyroid-stimulating hormone receptor activity protein binding sequence-specific double-stranded DNA binding transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding sequence-specific DNA binding
Process(es)
urogenital system development metanephros development branching involved in ureteric bud morphogenesis kidney development mesonephros development ventricular septum development mesenchymal to epithelial transition involved in metanephros morphogenesis transcription, DNA-templated regulation of transcription, DNA-templated regulation of transcription from RNA polymerase II promoter sulfur compound metabolic process nervous system development central nervous system development sensory organ development organ morphogenesis negative regulation of cardiac muscle cell apoptotic process cell differentiation thyroid gland development pronephric field specification inner ear morphogenesis regulation of apoptotic process positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter embryonic organ morphogenesis pronephros development ureteric bud morphogenesis cellular response to gonadotropin stimulus otic vesicle development S-shaped body morphogenesis kidney epithelium development positive regulation of mesenchymal to epithelial transition involved in metanephros morphogenesis mesonephric tubule development metanephric collecting duct development metanephric epithelium development metanephric distal convoluted tubule development metanephric nephron tubule development metanephric comma-shaped body morphogenesis metanephric S-shaped body morphogenesis metanephric nephron tubule formation positive regulation of metanephric DCT cell differentiation positive regulation of thyroid hormone generation regulation of thyroid-stimulating hormone secretion anatomical structure morphogenesis thyroid-stimulating hormone signaling pathway
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