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Invitrogen

CD3/CD4 Antibody Cocktail, FITC, PE

View all (3) CD3/CD4 antibodies

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Product Details

MA1-12264

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

Assay-dependent
-
Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG1

Class

Cocktail

Type

Antibody

Clone

M2AB, 7E14

Immunogen

human thymocytes and human peripheral blood T lymphocytes

Conjugate

FITC , PE FITC , PE FITC , PE

Form

Liquid

Purification

Protein A/G

Storage buffer

PBS with 0.2% carrier protein

Contains

0.08% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1071997

Product Specific Information

This antibody is a monoclonal mixture of FITC-conjugated CD3 (clone M2AB, isotype IgG1) and PE-conjugated CD4 (clone 7E14, isotype IgG1).

Target Information

CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The CD4 antigen is involved in the recognition of the type II MHC antigen. It is also a receptor for HIV. It is present on most T helper cells and normal thymocytes. The cytoplasmic portion of CD4 is associated with p56lck tyrosine kinase. CD4 expression is commonly found in human lymph nodes and tonsils. CD44 is a type 1 transmembrane glycoprotein also known as Phagocytic Glycoprotein-1 (pgp-1) and HCAM. CD44 is the receptor for hyaluronate and exists as a large number of different isoforms due to alternative RNA splicing. The major isoform expressed on lymphocytes, myeloid cells, and erythrocytes is a glycosylated type 1 transmembrane protein. Other isoforms contain glycosaminoglycans and are expressed on hematopoietic and non-hematopoietic cells. CD44 is involved in adhesion of leukocytes to endothelial cells, stromal cells, and the extracellular matrix.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: CD247; CD247 antigen, zeta subunit; CD3 antigen, delta subunit; CD3 delta; CD3-epsilon; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; CD4 antigen (p55); CD4 antigen p55; CD4 receptor; component of the TCR/CD3 complex; FLJ18683; Leu-2; MAL; OKT3, delta chain; OKT8 T-cell antigen; T cell co-receptor; T-cell antigen Leu2; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface antigen T4/Leu-3; T4 surface glycoprotein precursor; T8 T-cell antigen; TCR zeta; TCR zeta chain; tissue factor-targeting CAR1 dimer; tissue factor-targeting CAR1 monomer; unnamed protein product

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Gene Aliases: CD3-DELTA; CD3-GAMMA; CD3-ZETA; CD3DELTA; CD3epsilon; CD3GAMMA; CD3H; CD3Q; CD3Z; CD3ZETA; CD4mut; IMD17; IMD18; IMD19; IMD25; IMD79; Leu-3; OKT4D; T3D; T3E; T3G; T3Z; T4; TCRE; TCRZ

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UniProt ID: (Human) P20963, (Human) P04234, (Human) P07766, (Human) P09693, (Human) P01730

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Entrez Gene ID: (Human) 919, (Human) 915, (Human) 916, (Human) 917, (Human) 920

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Function(s)
transmembrane signaling receptor activity protein binding protein binding, bridging MHC class II receptor activity identical protein binding protein homodimerization activity protein heterodimerization activity protein tyrosine kinase binding SH3 domain binding protein kinase binding receptor signaling complex scaffold activity T cell receptor binding virus receptor activity receptor binding extracellular matrix structural constituent zinc ion binding lipid binding coreceptor activity immunoglobulin binding enzyme binding MHC class II protein complex binding signaling receptor activity interleukin-16 binding interleukin-16 receptor activity MHC class II protein binding
Process(es)
adaptive immune response cell surface receptor signaling pathway Fc-gamma receptor signaling pathway gamma-delta T cell activation alpha-beta T cell activation T cell receptor signaling pathway protein oligomerization protein-containing complex assembly positive thymic T cell selection establishment or maintenance of cell polarity protein transport T cell activation regulation of lymphocyte apoptotic process positive regulation of immune system process immune response cell adhesion signal transduction enzyme linked receptor protein signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway positive regulation of calcium ion transport into cytosol T cell differentiation macrophage differentiation leukocyte chemotaxis response to estradiol maintenance of protein location in cell positive regulation of interleukin-2 production response to vitamin D helper T cell enhancement of adaptive immune response interleukin-15-mediated signaling pathway positive regulation of T cell proliferation T cell selection response to ethanol positive regulation of monocyte differentiation viral entry into host cell positive regulation of inflammatory response regulation of T cell activation positive regulation of multicellular organismal process cellular response to granulocyte macrophage colony-stimulating factor stimulus response to methamphetamine hydrochloride cellular response to ionomycin positive regulation of cell-matrix adhesion signal complex assembly G-protein coupled receptor signaling pathway regulation of signal transduction positive regulation of gene expression negative regulation of gene expression dendrite development cerebellum development positive regulation of cell-cell adhesion mediated by integrin regulation of apoptotic process
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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