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Invitrogen
LAMP1 Monoclonal Antibody (LY1C6)
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FIGURE: 1 / 10
LAMP1 Antibody (MA1-164) in ICC/IF
Immunofluorescence analysis of LAMP1 (Lysosome-associated membrane glycoprotein 1) was performed using 80% confluent log phase L6 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with LAMP1 Monoclonal Antibody (LY1C6) (Product # MA1-164) at 10 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Ant... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-164
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Hamster,
Human,
Mouse,
Rat
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
LY1C6
Immunogen
Rat liver lysosomal membrane preparations
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA, 30% glycerol
Contains
0.05% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-164 detects glycosylated LAMP-1 at ~130kD.
M-PER (Product # 78501), IP Lysis Buffer (Product # 87787), or a 1% Triton X-100 lysis buffer are recommended for Western blot. LAMP-1 was not detected when RIPA buffer was used to generate cell lysates.
Target Information
LAMP1 (CD107a, lysosome-associated membrane protein-1) together with LAMP-2, is a major constituent of lysosomal membrane, 1-2% of total CD107a is found also on the plasma membrane. LAMP1 is a heavily glycosylated membrane protein which contains a putative signal peptide, 18 sites for N-linked glycosylation, a single membrane-spanning segment and a short (11 amino acid) cytosolic tail. The LAMP proteins are involved in lysosome biogenesis and are required for fusion of lysosomes with phagosomes. LAMP1 is a type 1 integral membrane protein that is transported from trans-Golgi network to endosomes and then lysosomes. Upon cell activation, LAMP1 transfer to the plasma membrane is dependent on a carboyxl-terminal tyrosine based motif (YXXI). Perturbation in the spacing between the tyrosine based motif relative to the membrane abolishes lysosome localization of LAMP1, and this mutant protein then cycles between the plasma membrane and the endosome. Cell surface LAMP1 (and LAMP2) have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium, therefore, they are possibly involved in the adhesion of PBMC to the site of inflammation. Increased LAMP1 immunoreactivity is observed in neurons and glial cells surrounding senile plaques in Alzheimer's Disease (AD) cases, and is localized in medullary epithelial cells, single macrophages and lymphocytes in acute thymic involution. LAMP1 is a good marker of mast cell activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 120 kDa lysosomal membrane glycoprotein; CD107 antigen-like family member A; Lamp I; LAMP-1; LGP-120; lysosomal associated membrane protein 1; Lysosomal associated membrane protein 1 (120 kDa); lysosomal membrane glycoprotein 1; lysosomal membrane glycoprotein A; lysosomal membrane glycoprotein-type A precursor; lysosomal membrane protein precursor; lysosomal-associated membrane protein 1; LYSOSOME-ASSOCIATED MEMBRANE GLYCOPROTEIN 1 PRECURSOR (LAMP-1) (LGP-A) (LGP-120) (CD107A) (P2B); Lysosome-associated membrane protein 1; membrane glycoprotein; P2B/LAMP-1; unnamed protein product
Gene Aliases: CD107a; I79_011073; LAMP-1; LAMPA; LGP-120; LGP-A; LGP120; Lgpa; P2B; Perk
UniProt ID: (Human) P11279, (Mouse) P11438, (Chinese hamster) P49129, (Rat) P14562
Entrez Gene ID: (Human) 3916, (Mouse) 16783, (Chinese hamster) 100689406, (Rat) 25328
lysosomal lumen acidification
positive regulation of natural killer cell degranulation
positive regulation of natural killer cell mediated cytotoxicity
protein stabilization
establishment of protein localization to organelle
Golgi to lysosome transport
granzyme-mediated programmed cell death signaling pathway
regulation of organelle transport along microtubule
spermatogenesis
lysosomal lumen pH elevation
autophagic cell death
hydrogen ion transmembrane transport
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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