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Invitrogen

Caspase 8 Monoclonal Antibody (FLICE 4-1-20)

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Caspase 8 Monoclonal Antibody (FLICE 4-1-20)

Product Details

MA1-16841

Applications
Tested Dilution
Publications

Western Blot (WB)

1:1,000
-
Product Specifications

Species Reactivity

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Class

Monoclonal

Type

Antibody

Clone

FLICE 4-1-20

Immunogen

Full-length recombinant human Caspase 8.
3D Epitope / Immunogen

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.1% sodium azide

Storage conditions

4°C, do not freeze

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_568481

Product Specific Information

Suggested positive control: antigen standard for CASP8 (transient overexpression lysate), HEK 293 whole cell lysate.

Target Information

Caspase 8 (CASP8) is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases play a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes composed of a pro-domain, a large protease subunit, and a small protease subunit. Activation of caspases requires proteolytic processing at conserved internal aspartic residues to generate a heterodimeric enzyme consisting of the large and small subunits. Caspase 8 is involved in the programmed cell death induced by Fas and various apoptotic stimuli. The N-terminal FADD-like death effector domain of Caspase 8 suggests that it may interact with Fas-interacting protein FADD. Caspase 8 was detected in the insoluble fraction of the affected brain region from Huntington disease patients but not in those from normal controls, which implicated the role in neurodegenerative diseases. Caspase 8 binds to the death effector domain (DED) of FADD through an analogous DED domain present in tandem in the pro-form of the Caspase 8 protein. Activated Caspase 8 then activates other downstream caspases including Caspase 9, thereby committing the cell to undergo apoptosis. In addition, Caspase 8 also reacts with Jurkat cells and Tonsil. Overexpression of Caspase 8 induces apoptosis, which can be blocked by inhibitors specific for the ICE family. Many alternatively spliced transcript variants encoding different isoforms have been described for Caspase 8, however, not all variants have had their full-length sequences determined.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: apoptotic cysteine protease; apoptotic protease Mch-5; Casp8; caspase 8, apoptosis-related cysteine peptidase; caspase 8, apoptosis-related cysteine protease; caspase precursor; Caspase-8 precursor; caspase-8s; Caspase8; EC 3.4.22.61; FADD-homologous ICE/CED-3-like protease; FADD-like ICE; Fas-linked ICE-like protease; flice; FLJ17672; ICE-like apoptotic protease 5; ICE8; MACH-alpha-1/2/3 protein; MACH-beta-1/2/3/4 protein; MGC78473; MORT1-associated ced-3 homolog; OTTHUMP00000163720; OTTHUMP00000165063; OTTHUMP00000206557; OTTHUMP00000206581; unnamed protein product

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Gene Aliases: ALPS2B; CAP4; CASP-8; FLICE; MACH; MCH5

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UniProt ID: (Human) Q14790, (Mouse) O89110

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Entrez Gene ID: (Human) 841, (Mouse) 12370

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Function(s)
endopeptidase activity cysteine-type endopeptidase activity death receptor binding protein binding peptidase activity cysteine-type peptidase activity cysteine-type endopeptidase activator activity involved in apoptotic process ubiquitin protein ligase binding death effector domain binding identical protein binding macromolecular complex binding scaffold protein binding tumor necrosis factor receptor binding
Process(es)
angiogenesis regulation of cytokine production neural tube formation regulation of immune system process proteolysis apoptotic process heart development extrinsic apoptotic signaling pathway via death domain receptors positive regulation of signal transduction protein processing cell differentiation protein catabolic process macrophage differentiation positive regulation of cell migration positive regulation of interleukin-1 beta production response to tumor necrosis factor TRAIL-activated apoptotic signaling pathway regulation of apoptotic process chordate embryonic development positive regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of neuron apoptotic process regulation of innate immune response response to ethanol positive regulation of macrophage differentiation positive regulation of proteolysis animal organ development cardiac muscle tissue development protein maturation negative regulation of necroptotic process regulation of thymocyte apoptotic process pyroptosis cellular response to mechanical stimulus apoptotic signaling pathway extrinsic apoptotic signaling pathway execution phase of apoptosis self proteolysis hepatocyte apoptotic process positive regulation of pyroptotic inflammatory response positive regulation of execution phase of apoptosis regulation of apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway regulation of tumor necrosis factor-mediated signaling pathway natural killer cell activation response to cobalt ion response to estradiol response to lipopolysaccharide T cell activation B cell activation syncytiotrophoblast cell differentiation involved in labyrinthine layer development death-inducing signaling complex assembly response to anesthetic
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