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Invitrogen
alpha Tubulin Monoclonal Antibody (TU-01)
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-19162
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Many,
Mouse,
Pig
Published species
Bovine,
Deer,
Human,
Mouse,
Non-human primate,
Pig,
Protozoa,
Rat,
Xenopus
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
TU-01
Immunogen
Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody recognizes a defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin.
HPB-ALL human peripheral blood leukemia cell line is the suggested positive control (incubate for 90 mins).
Antibodies to this protein (and modification) were previously sold as part of a Thermo Scientific Cellomics High Content Screening Kit. This replacement antibody is now recommended for researchers who need an antibody for high content cell based assays. It has been thoroughly tested and validated for cellular immunofluorescence (IF) applications. Further optimization including the selection of the most appropriate fluorescent Dylight conjugated secondary antibody may have to be performed for your high content assay.
Western Blot: Reducing conditions.
Target Information
Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 4 tubulin; alpha tubulin; alpha-1 tubulin; alpha-2; alpha-tubulin; Alpha-tubulin 1; alpha-tubulin 2; alpha-tubulin 3C; alpha-tubulin 3C/D; Alpha-tubulin 3D; alpha-tubulin 6; alpha-tubulin isotype M-alpha-1; alpha-tubulin isotype M-alpha-2; alpha-tubulin isotype M-alpha-4; alpha-tubulin isotype M-alpha-6; alpha-tubulin ubiquitous; class II beta-tubulin isotype; class IIb beta-tubulin; epididymis secretory sperm binding protein; FLJ30169; gangliocyte protein related to virus stimulation; H2 ALPHA; hum-a-tub1; hum-a-tub2; MGC8685; similar to beta tubulin; Talpha1 alpha-tubulin; TUA; TUA1; TUA2; TUA3; TUBA1/3/4; Tubulin alpha; tubulin alpha 3; tubulin alpha-1; Tubulin alpha-1 chain; tubulin alpha-2 chain; tubulin alpha-3; tubulin alpha-3 chain; tubulin alpha-3C/D chain; Tubulin alpha-3D chain; tubulin alpha-4 chain; tubulin alpha-6 chain; tubulin alpha-ubiquitous chain; tubulin B-alpha-1; tubulin H2-alpha; tubulin K-alpha-1; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 2; tubulin, alpha 6; tubulin, alpha, brain-specific; tubulin, alpha, ubiquitous; tubulin, beta 2B; tubulin, beta polypeptide paralog; unnamed protein product
Gene Aliases: 2410129E14Rik; ALS22; B-ALPHA-1; bA408E5.3; bA506K6.1; bcm948; brdp; CDCBM7; CMYO26; FTDALS9; H2-ALPHA; K-ALPHA-1; LIS3; M[a]4; M[a]6; OZEMA23; OZEMA24; PMGYSA; SPAX11; Tuba-1; TUBA1; TUBA2; TUBA3; Tuba4; TUBA6
UniProt ID: (Human) Q71U36, (Human) P68363, (Pig) Q2XVP4, (Human) Q9BQE3, (Human) P0DPH7, (Human) Q6PEY2, (Human) P68366, (Human) A6NHL2, (Human) Q9BVA1, (Mouse) P68369, (Mouse) P05213, (Mouse) P68373, (Mouse) P68368, (Mouse) Q3UX10, (Mouse) Q9CWF2
Entrez Gene ID: (Pig) 100135051, (Human) 7846, (Human) 10376, (Pig) 733594, (Human) 84790, (Human) 7278, (Human) 112714, (Human) 7277, (Pig) 100151951, (Human) 79861, (Pig) 100623355, (Human) 347733, (Pig) 100153507, (Mouse) 22142, (Mouse) 22143, (Mouse) 22146, (Mouse) 22145, (Mouse) 238463, (Mouse) 73710
GTPase activity
structural molecule activity
structural constituent of cytoskeleton
protein binding
GTP binding
microtubule binding
identical protein binding
macromolecular complex binding
protein heterodimerization activity
chaperone binding
double-stranded RNA binding
ubiquitin protein ligase binding
enzyme binding
protein kinase binding
protein domain specific binding
tubulin
microtubule cytoskeleton organization
mitotic cell cycle
neuron migration
startle response
protein folding
'de novo' protein folding
intracellular protein transport
microtubule-based process
centrosome cycle
smoothened signaling pathway
memory
locomotory behavior
adult locomotory behavior
visual learning
response to mechanical stimulus
glial cell differentiation
gene expression
dentate gyrus development
cerebellar cortex morphogenesis
hippocampus development
pyramidal neuron differentiation
cerebral cortex development
neurogenesis
neuron differentiation
sperm motility
adult behavior
cytoskeleton-dependent intracellular transport
response to tumor necrosis factor
locomotory exploration behavior
microtubule polymerization
forebrain morphogenesis
homeostasis of number of cells within a tissue
regulation of synapse organization
synapse organization
protein stabilization
cell division
neuron apoptotic process
motor behavior
cellular response to calcium ion
organelle transport along microtubule
neuron projection arborization
response to L-glutamate
cellular response to interleukin-4
cytoskeleton organization
positive regulation of axon guidance
modulation of synaptic transmission
embryonic brain development
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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