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Invitrogen
HLA-E Monoclonal Antibody (MEM-E/02)
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FIGURE: 1 / 1
HLA-E Antibody (MA1-19309) in Flow
Flow Cytometry analysis of HLA-E using HLA-E Monoclonal Antibody (MEM-E/02) (Product # MA1-19309). Anti-Hu HLA-E purified antibody (concentration in sample 5 μg/mL, red-filled histogram) binds specifically to surface HLA-E on surface of HLA-E transfected LCL 721.221 cells, but not to RAJI cells, K562 cells, HLA-G transfected LCL 721.221 cells and non-transfected LCL 721.221 cells. Level of non-specific binding was assessed using Mouse IgG1 isotype control PE under same conditions (concentration in sample 5 μg/mL, black-dashed histogram).
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-19309
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
MEM-E/02
Immunogen
Recombinant human HLA-E denaturated heavy chain.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS, pH 7.4
Contains
15mM sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody reacts with an extracellular epitope on denaturated heavy chain of human HLA-E. HLA-E belongs to the MHC Class I molecules (MHC Class Ib; nonclassical) and it is expressed on the surface of all human cell types.
Immunohistochemistry (Paraffin): Heat antigen retrieval (sodium citrate), incubation with mAb: 1 hour / RT, detection DAB.
Target Information
HLA-E belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. HLA-E binds a restricted subset of peptides derived from the leader peptides of other class I molecules. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region, and exons 6 and 7 encode the cytoplasmic tail.
HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: DAMA-277I14.1; DAQB-90C11.16; DKFZp686P19218; FLJ26655; HLA-E class I protein precursor; MHC 1; MHC class I antigen E; MHC class Ib antigen; MHC1; non-classical MHC class I antigen; nonclassical MHC class I antigen; unnamed protein product
Gene Aliases: HLA-6.2; QA1
UniProt ID: (Human) P13747
Entrez Gene ID: (Human) 3133
positive regulation of antibody-dependent cellular cytotoxicity
positive regulation of T cell mediated cytotoxicity
adaptive immune response
antigen processing and presentation of endogenous peptide antigen via MHC class Ib
antigen processing and presentation of exogenous peptide antigen via MHC class Ib
antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-independent
natural killer cell tolerance induction
positive regulation of immunoglobulin production
regulation of natural killer cell mediated immunity
positive regulation of natural killer cell mediated immunity
positive regulation of natural killer cell cytokine production
immune response
signal transduction
antibacterial humoral response
antigen processing and presentation
positive regulation of interleukin-13 production
positive regulation of interleukin-4 production
positive regulation of TRAIL production
positive regulation of tumor necrosis factor production
negative regulation of natural killer cell activation
positive regulation of natural killer cell activation
positive regulation of natural killer cell proliferation
CD8-positive, alpha-beta T cell activation
negative regulation of T cell proliferation
protection from natural killer cell mediated cytotoxicity
negative regulation of natural killer cell mediated cytotoxicity
positive regulation of natural killer cell mediated cytotoxicity
defense response to Gram-positive bacterium
positive regulation of CD8-positive, alpha-beta T cell proliferation
positive regulation of CD8-positive, alpha-beta T cell activation
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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