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Invitrogen
Mouse anti-Human Kappa Light Chain Secondary Antibody
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FIGURE: 1 / 5
Human Kappa Light Chain Secondary Antibody (MA1-35486) in WB
Western blot was performed using Human IgG kappa Gatipotuzumab Recombinant Human Monoclonal Antibody (Product # MA5-42157) (Fig. a) and Human IgG lambda Anetumab Recombinant Human Monoclonal Antibody (Product # MA5-42007) (Fig. b). Whole cell lysates of T-47D (Fig. a Lane 1-3), MCF7 (Fig. a Lane 4-6), NIH:OVCAR-3 (Fig. b Lane 1-3) and PC-3 (Fig. b Lane 4-6) were electrophoresed using NuPAGE™ 3-8% Tris-Aceta... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-35486
Applications
Tested Dilution
Western Blot (WB)
1:2000
Immunohistochemistry (Frozen) (IHC (F))
1:30
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Secondary Antibody
Clone
2B7
Immunogen
Kappa Bence Jones protein
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
10 µg/mL
Amount
10 µg
Storage buffer
tissue culture supernatant
Contains
10mM sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Target
Kappa light chain
Antibody Form
Whole Antibody
Product Specific Information
The antibody reacts with free kappa chains, but not with kappa chains attached to heavy chains. No reaction is observed with Ig kappa on membranes of lymphocytes. No crossreactivity with lambda chains. In Ouchterlony immunodiffusion no reaction with Bence Jones kappa is observed.
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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