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MYOD Monoclonal Antibody (5.8A)
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FIGURE: 1 / 23
MYOD Antibody (MA1-41017) in ICC/IF
Immunofluorescence analysis of MYOD was performed using 70% confluent log phase differentiated C2C12 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with MYOD monoclonal antibody (Product # MA1-41017) at 5 µg/mL dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L), Superclonal™ Recombinant Secondary Antibody, Alexa Fluor 488 conjuga... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-41017
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Dog,
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
5.8A
Immunogen
Recombinant mouse MyoD protein.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.0 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.02% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The epitope of this antibody was mapped to a region within AA 180-189 of mouse MyoD.
Suggested positive control: Rh-30.
Target Information
The Myogenic determination gene (MyoD) was first identified by the virtue of its ability to convert embryonic mouse fibroblast cells to muscle cells. It was subsequently shown that forced expression of MyoD (human homolog is myf 3) gene in a wide variety of normal and neoplastic cells could either convert the cells to muscle cells or activate a set of the otherwise transcriptionally inactive muscle-specific genes in these cells. The regulatory domain of the MyoD gene product lies within a 70 amino acid region and comprises a basic DNA binding motif and a helix-loop-helix (HLH) dimerization motif. Subsequent studies identified three other genes whose products shared sequence homology for the basic HLH domain of MyoD. These are; myf5, myogenin (human homolog is myf4) and myf6 (also known as MRF4 and herculin).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: class C basic helix-loop-helix protein 1; myf-3; MyoD; MyoD1; myogenic factor 3; unnamed protein product
Gene Aliases: bHLHc1; CMYO17; CMYP17; MYF3; MYOD; Myod-1; MYODRIF; PUM
UniProt ID: (Human) P15172, (Mouse) P10085
Entrez Gene ID: (Human) 4654, (Mouse) 17927
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
bacterial-type RNA polymerase transcriptional activator activity, sequence-specific DNA binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
chromatin binding
transcription factor activity, sequence-specific DNA binding
protein binding
ligand-dependent nuclear receptor binding
enzyme binding
chromatin DNA binding
ubiquitin protein ligase binding
protein homodimerization activity
bHLH transcription factor binding
sequence-specific DNA binding
protein dimerization activity
RNA polymerase II sequence-specific DNA binding transcription factor binding
E-box binding
sequence-specific double-stranded DNA binding
promoter-specific chromatin binding
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
muscle organ development
skeletal muscle tissue development
tissue development
regulation of gene expression
myotube cell development
skeletal muscle cell differentiation
muscle cell differentiation
muscle cell fate commitment
protein unfolding
skeletal muscle tissue regeneration
regulation of RNA splicing
positive regulation of myoblast differentiation
positive regulation of transcription from RNA polymerase II promoter
animal organ development
positive regulation of skeletal muscle fiber development
positive regulation of muscle cell differentiation
cellular response to estradiol stimulus
positive regulation of myoblast fusion
positive regulation of snRNA transcription by RNA polymerase II
regulation of alternative mRNA splicing, via spliceosome
myoblast fate determination
myoblast fusion
cellular response to starvation
myotube differentiation
myotube differentiation involved in skeletal muscle regeneration
positive regulation of skeletal muscle tissue regeneration
skeletal muscle fiber adaptation
myoblast differentiation
positive regulation of transcription, DNA-templated
skeletal muscle fiber development
striated muscle cell differentiation
cardiac muscle cell differentiation
cellular response to tumor necrosis factor
cellular response to glucocorticoid stimulus
cellular response to oxygen levels
negative regulation of myoblast proliferation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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