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Invitrogen
Progesterone Receptor Monoclonal Antibody (Alpha PR6)
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FIGURE: 1 / 25
Progesterone Receptor Antibody (MA1-411) in ICC/IF
Immunofluorescent analysis of Progesterone Receptor (green) in T47D cells untreated (left) or stimulated with 100nm promegestone, R5020, for 1 hour (right). The cells were fixed with formalin for 15 minutes, permeabilized with 0.1% Triton X-100 in TBS for 10 minutes, and blocked with 3% Blocker BSA (Product # 37525) for 15 minutes at room temperature. Cells were stained with Progesterone Receptor mouse mono... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-411
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Chicken,
Guinea pig,
Human,
Mouse,
Sheep,
Rabbit,
Rat
Published species
Camelid,
Chicken,
Human,
Mouse,
Non-human primate,
Rat,
Sheep
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
Alpha PR6
Immunogen
Progesterone receptor purified from chick oviduct cytosol.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-411 reacts with high affinity to the B form of the progesterone receptor from human, mouse, rat, rabbit, bovine, ovine, chicken, and guinea pig tissues. This antibody does not cross-react with estrogen receptor or glucocorticoid receptor.
MA1-411 has been successfully used in Western blot, immunocytochemical, immunohistochemical and immunoprecipitation procedures. By Western blot, this antibody detects a 110 kDa protein representing the B form of the PR in chickens."
The MA1-411 immunogen is progesterone receptor purified from chick oviduct cytosol.
Reconstitute with PBS.
Target Information
The progesterone receptor (PR) is a member of the steroid family of nuclear receptors. The PR mediates the physiological effects of progesterone, which plays a central role in reproductive events associated with the establishment and maintenance of pregnancy. PR is found as a 94 kDa protein (Form A) or a 120 kDa protein (Form B) due to the use of alternative translation initiation sites. PR-B is the transcriptionally active form and is responsible for activating genes for the maintenance of the endometrium, maintenance of pregnancy, and inhibition of ovulation. PR-A is identical to PR-B except for a 165 amino acid deletion at the N-terminus. This deletion exposes a 140 amino acid inhibitory domain (ID) that acts as a repressor of steroid hormone transcriptional activity. In its inactive state, PgR forms a multiprotein complex which includes heat shock proteins and immunophins. Upon binding of progesterone hormone to its receptor, there is a conformational change that allows dimerization and binding of the receptor to progesterone response elements (PRE) sequences, resulting in activated transcription. A Null mutation in the PGR gene leads to pleiotrophic reproductive abnormalities.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: nuclear receptor subfamily 3 group C member 3; pot. alt. progesterone receptor; putative; PR; progesterone receptor; progesterone receptor A; progesterone-receptor; steroid hormone receptor; unnamed protein product
Gene Aliases: 9930019P03Rik; NR3C3; PR; PR-A; PR-B
UniProt ID: (Rabbit) P06186, (Chicken) P07812, (Human) P06401, (Mouse) Q00175, (Rat) Q63449
RNA polymerase II core promoter proximal region sequence-specific DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
zinc ion binding
enzyme binding
Hsp70 protein binding
chromatin DNA binding
sequence-specific DNA binding
Hsp90 protein binding
steroid hormone binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription coactivator binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
receptor binding
steroid binding
protein binding
estrogen response element binding
identical protein binding
ATPase binding
sequence-specific double-stranded DNA binding
transcription regulatory region sequence-specific DNA binding
hormone binding
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
intracellular steroid hormone receptor signaling pathway
response to progesterone
steroid hormone mediated signaling pathway
response to estrogen
signal transduction
cell-cell signaling
positive regulation of gene expression
negative regulation of gene expression
negative regulation of phosphorylation
positive regulation of transcription from RNA polymerase II promoter
progesterone receptor signaling pathway
maintenance of protein location in nucleus
ovulation from ovarian follicle
epithelial cell maturation
positive regulation of cell proliferation
female gonad development
positive regulation of smooth muscle cell migration
ovulation cycle process
mammary gland development
paracrine signaling
response to cocaine
negative regulation of apoptotic process
positive regulation of transcription, DNA-templated
lung alveolus development
regulation of epithelial cell proliferation
female mating behavior
tertiary branching involved in mammary gland duct morphogenesis
negative regulation of granulosa cell apoptotic process
luteinization
diterpenoid metabolic process
estrous cycle
negative regulation of transcription, DNA-templated
smooth muscle relaxation of the bladder outlet
cellular response to gonadotropin stimulus
cellular response to follicle-stimulating hormone stimulus
cellular response to luteinizing hormone stimulus
cellular response to estrogen stimulus
response to ketone
cellular response to wortmannin
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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