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Invitrogen
6x-His Tag Monoclonal Antibody (13/45/31-2)
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FIGURE: 1 / 3
6x-His Tag Antibody (MA1-4806) in ICC/IF
Immunofluorescence detection of (HIS)6-GFP in Hela-cells transfected with expression plasmid CMV-His6-GFP-APKD1-5a. LEFT: GFP autofluorescence; RIGHT: 1:100 anti-HIS (Product # MA1-4806) (in red) and Cy3-labeled goat anti-mouses secondary antibody.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-4806
Applications
Tested Dilution
Publications
Product Specifications
Published species
Tag
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
13/45/31-2
Immunogen
Recombinant (His) 6-p53 protein.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
200 µg
Storage conditions
4°C, do not freeze
Shipping conditions
Wet ice
RRID
Product Specific Information
MA1-4806 detects any kind of 6X-His tagged proteins in cells and complex cellular lysates.
MA1-4806 has been successfully used in flow cytometry, immunohistochemistry (frozen), immunofluorescence, Western blot, immunoprecipitation, and ELISA procedures. By Western blot this antibody detects recombinant proteins containing an epitope of at least 6 histidine residues, located at the N-terminus, C-terminus or at both termini. A higher number of histidine residues leads to an increased binding affinity of the antibody.
The MA1-4806 immunogen is recombinant (His) 6-p53 protein
Target Information
The 6x His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). The His tag is commonly expressed as a tag at either N-or C-terminal regions of recombinant proteins to allow isolation or purification by immobilized metal affinity chromatography. Epitope tagging is a technique in which a known epitope is fused to a recombinant protein using genetic engineering. By choosing a particular epitope and recombinant protein combination, epitope tagging makes it possible to detect proteins for which no antibody is available. His epitope tagged proteins that contain a stretch of histidine residues at the carboxyl or amino terminus are used for purification of recombinant proteins by means of metal chelate chromatography due to metal binding properties of histidine clusters on His tagged proteins. For example, the affinity of the histidine-tag motif to Ni2+ by chelation is strong and selective enough to enable purification of the protein to homogeneity by affinity chromatography on a Ni2+-NTA adsorbent. His tag-specific antibodies are used to facilitate detection or coimmunopreciptation of tagged proteins.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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