Search
Invitrogen
Prolactin Receptor Monoclonal Antibody (U5)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 11
Prolactin Receptor Antibody (MA1-610) in IHC
Mammary gland tissue from lactating light horse mare (equine) was incubated in MA1-610 anti-prolactin receptor monoclonal mouse antibody at 1:100 for 1 hour at 23°C and for 24 hours at 4°C. Second antibody was biotinylated goat anti-mouse IgG at 23°C for 1 hour. Development using avidin-biotin complex 23°C for 1 hour plus 3, 3-diaminobenzadine for 15 min and counterstained with haematoxylin. Notes: Antigen ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-610
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Horse,
Human,
Mouse,
Pig,
Rabbit,
Rat
Published species
Baboon,
Bovine,
Hamster,
Human,
Mouse,
Non-human primate,
Pig,
Rabbit,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
U5
Immunogen
Purified rat liver PRL receptor.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Lyophilized
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-610 detects prolactin (PRL) receptor from rat, human, rabbit, horse, and pig tissues.
MA1-610 has been successfully used in Western blot, immunohistochemistry, immunocytochemistry, immunoprecipitation, immunofluorescence, and FACS procedures. By Western blot, this antibody detects an ~40 kDa protein and an ~100 kDa protein representing both the short and long forms of the PRL receptor, respectively, in various tissues. Immunohistochemical staining of PRL receptor in rat Islets of Langerhans with MA1-610 results in a staining pattern consistent with cytoplasmic vesicle staining. MA1-610, after biotinylation, has been used to detect human lymphocyte PRL receptor by FACS. MA1-610 does not inhibit the interaction of prolactin with the receptor.
The MA1-610 immunogen is purified PRL receptor from rat liver. This antibody has been shown to react with the extracellular portion of the receptor, but not in the ligand binding domain. MA1-610 does not inhibit the interaction of prolactin with the receptor.
Reconstitute with 100 µL PBS.
Target Information
Prolactin (PRL) is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor (PRLR), a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40 kDa to 100 kDa. PRLR consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cytokine receptor; cytokine receptor; PRLRSFA; cytokine receptor; PRLRSFB; delta-S1 PRLr; hPRL receptor; lactogen receptor; lactogen receptor precursor; Prl R; PRL-R; prolactin receptor; prolactin receptor 2 precursor; prolactin receptor precursor; prolactin receptor related sequence 1; prolactin receptor short isoform; pseudo-prolactin receptor precursor; secreted prolactin binding protein; unnamed protein product
Gene Aliases: HPRL; hPRLrI; MFAB; pPRLR-SF; Pr-1; Pr-3; Prlr-rs1; RATPRLR; RI-PRLR
UniProt ID: (Rabbit) P14787, (Pig) Q6JTA8, (Human) P16471, (Rat) P05710, (Mouse) Q08501
Entrez Gene ID: (Rabbit) 100009046, (Pig) 414916, (Horse) 100053793, (Human) 5618, (Rat) 24684, (Mouse) 19116
prolactin signaling pathway
oncostatin-M-mediated signaling pathway
leukemia inhibitory factor signaling pathway
steroid biosynthetic process
activation of transmembrane receptor protein tyrosine kinase activity
embryo implantation
lactation
positive regulation of cell proliferation
cytokine-mediated signaling pathway
positive regulation of B cell proliferation
activation of Janus kinase activity
negative regulation of apoptotic process
cellular response to granulocyte macrophage colony-stimulating factor stimulus
positive regulation of cold-induced thermogenesis
JAK-STAT cascade
response to bacterium
regulation of cell adhesion
regulation of epithelial cell differentiation
positive regulation of protein autophosphorylation
mammary gland epithelial cell differentiation
prostate gland growth
mammary gland alveolus development
mammary gland epithelium development
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support