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NR3C2 Monoclonal Antibody (H10E4C9F)
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FIGURE: 1 / 10
NR3C2 Antibody (MA1-620) in ICC/IF
Immunofluorescent analysis of Mineralocorticoid Receptor using Mineralocorticoid Receptor Monoclonal antibody (H10E4C9F) (Product # MA1-620) shows staining in HEK293 cells. Mineralocorticoid Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Mineralocorticoid Receptor (Product # MA1-620) at a dilution of 1:20-1:200 over night at 4 °C, wa... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA1-620
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Chicken,
Human,
Rabbit,
Rat
Published species
Chicken,
Human,
Mouse,
Rabbit,
Rat,
Sheep
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
H10E4C9F
Immunogen
Aldosterone-3
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.4 mg/mL
Amount
140 µg
Purification
Protein A
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA1-620 detects mineralocorticoid receptor (MR) in human, rat, rabbit and chicken tissues.
MA1-620 has been successfully used in Western blot, immunocytochemistry and immunohistochemistry procedures. By Western blot, this antibody detects a 116 kDa protein representing MR in rat heart homogenate. Immunohistochemical staining of MR in rabbit atrium with MA1-620 results in strong staining of myocytes and endothelial cells. MA1-620 cannot be used for immunoprecipitation.
In immunohistochemical studies, staining with MA1-620 is blocked by pre-incubating the sample with aldosterone. MA1-620 can also be used in formalin-fixed, paraffin-embedded sections. Due to the extremely low levels of MR expressed in native tissues, it is recommended that enhanced detection systems be used for Western blotting such as enhanced chemiluminescence. MA1-620 blocks the binding of aldosterone since it is directed against the steroid binding domain of the mineralocorticoid receptor. The antibody can also react with the steroid binding domain for aldosterone on other receptors.
The MA1-620 immunogen is aldosterone-3. This antibody was produced using the anti-idiotypic method.
Target Information
Steroid receptors are ligand-dependent, intracellular proteins that stimulate transcription of specific genes by binding to specific DNA sequences following activation by the appropriate hormone. Mineralocorticoids are a family of steroids, secreted by the adrenal cortex, necessary for the regulation of a number of metabolic processes including electrolyte regulation. These compounds exert their effect through their interaction with the mineralocorticoid receptor (MR) and that complex's subsequent association with DNA. Given the function of mineralocorticoids, it is not surprising to find that the kidney is a primary target organ for mineralocorticoids and that this organ has been shown to contain MR. The corresponding gene for the mineralocorticoid receptor is NR3C2.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: aldosterone receptor; mineralocorticoid receptor; Mineralocorticoid receptor (aldosterone receptor); mineralocorticoid receptor 1; mineralocorticoid receptor 2; mineralocorticoid receptor delta; MR; nuclear hormone receptor; unnamed protein product
Gene Aliases: MCR; MLR; MR; NR3C2VIT
UniProt ID: (Human) P08235, (Rat) P22199
Entrez Gene ID: (Chicken) 374131, (Rabbit) 100358449, (Human) 4306, (Rat) 25672
RNA polymerase II core promoter proximal region sequence-specific DNA binding
DNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
steroid binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcription factor activity, sequence-specific DNA binding
steroid hormone receptor activity
protein binding
zinc ion binding
TBP-class protein binding
estrogen response element binding
sequence-specific DNA binding
sequence-specific double-stranded DNA binding
transcription coactivator binding
double-stranded DNA binding
histone acetyltransferase binding
identical protein binding
macromolecular complex binding
regulation of transcription from RNA polymerase II promoter
intracellular steroid hormone receptor signaling pathway
regulation of blood volume by renal aldosterone
regulation of transcription, DNA-templated
cellular sodium ion homeostasis
signal transduction
locomotory behavior
nuclear receptor-mediated corticosteroid signaling pathway
renal sodium excretion
angiotensin-activated signaling pathway
regulation of cell proliferation
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
response to corticosterone
potassium ion homeostasis
regulation of postsynaptic membrane potential
response to alcohol
presynaptic modulation of chemical synaptic transmission
positive regulation of NIK/NF-kappaB signaling
response to ketone
cellular response to aldosterone
regulation of potassium:proton exchanging ATPase activity
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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