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Invitrogen
HSP90 alpha Monoclonal Antibody (3G3)
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FIGURE: 1 / 11
HSP90 alpha Antibody (MA3-011) in ICC/IF
Immunofluorescent analysis of Heat Shock Protein 90 using Heat Shock Protein 90 Monoclonal antibody (3G3) (Product # MA3-011) shows staining in WiDr colon carcinoma cells. Heat Shock Protein 90 staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA3-011
Applications
Tested Dilution
Publications
Product Specifications
Published species
Fruit fly,
Human,
Mouse,
Non-human primate,
Rabbit,
Rat,
Virus
Host/Isotype
Mouse
/ IgM
Class
Monoclonal
Type
Antibody
Clone
3G3
Immunogen
Purified mouse HSP90.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-011 detects heat shock protein 90 kDa (HSP90) from human, mouse, rat, rainbow trout, and chicken tissues.
MA3-011 has been successfully used in Western blot, immunofluorescence, immunohistochemistry, and immunoprecipitation procedures. By Western blot, this antibody detects a 90 kDa protein representing HSP90 from Hepa 1 cell extract. It has been reported that up to 90% of cytosolic HSP90 can be immunoprecipitated with this product under low ionic strength conditions.
For Western blots using MA3-011, nitrocellulose is recommended. PVDF membranes typically do not yield acceptable results with this antibody.
Before use, centrifuge suspension (4000 g for 15 minutes) and discard supernatant. Resuspend pellet in 200 µL of PBS, pH 7.2. If desired, 0.05% sodium azide may be added to resuspended solution. Removal of residual ammonium sulfate is not usually necessary for immunoprecipitation and Western blot procedures, however, any other uses may require its removal by dialysis or equivalent method. Stable for 6 months after PBS resuspension.
The MA3-011 antigen is purified HSP90 from Hepa 1 cytosol.
Target Information
Heat shock proteins (HSP) are proteins that are expressed in response to various biological stress, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, and some kinases. It has been suggested that HSP90 is necessary to maintain structural integrity of at least some steroid receptors to allow for ligand binding during receptor activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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