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          • Primary Antibodies ›
          • uNOS Antibodies

          Invitrogen

          uNOS Monoclonal Antibody (NOS-3F7-B11 B5)

          2 Published Figures
          10 References
          View all (3) uNOS antibodies

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          Cite uNOS Monoclonal Antibody (NOS-3F7-B11 B5)

          Additional Information:

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          • Antibody Testing Data (5)
          • Published Figures (2)
          uNOS Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          uNOS Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          FIGURE: 1 / 7

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          uNOS Antibody (MA3-030) in ICC/IF

          Immunofluorescent analysis of uNOS (green) in SK-N-MC cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes at room temperature and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a uNOS monoclonal antibody (NOS-3F7-B11 B5) (Product # MA3-030) at a dilution of 1:20 and incubated overnight in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody for 45 minutes at room temperature in the dark. F-actin (red) was st... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
          Published figure supplied by benchsci-logo
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          View Product

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          uNOS Antibody in Immunocytochemistry (ICC/IF)
          uNOS Antibody in Immunocytochemistry (ICC/IF)
          uNOS Antibody in Immunocytochemistry (ICC/IF)
          uNOS Antibody in Immunocytochemistry (ICC/IF)
          uNOS Antibody in Western Blot (WB)
          uNOS Antibody in Western Blot (WB)
          uNOS Antibody in Western Blot (WB)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          Product Details

          MA3-030

          Applications
          Tested Dilution
          Publications

          Western Blot (WB)

          1:50-1:500
          View 2 publications 2 publications

          Immunohistochemistry (IHC)

          -
          View 8 publications 8 publications

          Immunohistochemistry (Frozen) (IHC (F))

          1:100
          -

          Immunocytochemistry (ICC/IF)

          1:20
          -

          Immunoprecipitation (IP)

          Assay-dependent
          -
          Product Specifications

          Species Reactivity

          Bovine, Human, Mouse, Rat

          Published species

          Human, Mouse, Rat

          Host/Isotype

          Mouse / IgM

          Class

          Monoclonal

          Type

          Antibody

          Clone

          NOS-3F7-B11 B5

          Immunogen

          Purified bovine bNOS.

          Conjugate

          Unconjugated Unconjugated Unconjugated

          Form

          Liquid

          Concentration

          Conc. Not Determined

          Storage buffer

          ascites

          Contains

          0.05% sodium azide

          Storage conditions

          -20°C, Avoid Freeze/Thaw Cycles

          Shipping conditions

          Ambient (domestic); Wet ice (international)

          RRID

          AB_325476

          Product Specific Information

          MA3-030 detects brain nitric oxide synthase (bNOS), inducible NOS (iNOS) and epithelial NOS (eNOS) in bovine, human, mouse and rat tissues.

          MA3-030 has been successfully used in Western blot, immunofluorescence, immunoprecipitation, and immunohistochemical experiments. By Western blot, this antibody detects an ~130 kDa band representing iNOS in samples first induced with interferon (IFN) and lipopolysaccarides (LPS), an ~155 kDa band in tissues expressing bNOS and an ~140 kDa band in tissues expressing eNOS. Immunohistochemical staining of NOS with MA3-030 yields a pattern consistent with that seen in the literature and depends on the tissue being studied and the localization of the isoforms present.

          The MA3-030 antigen is purified bovine bNOS.

          Target Information

          NOS oxidizes a guanidine nitrogen of arginine releasing nitric oxide in the form of a free radical and citrulline. Nitric oxide thus generated acts as a messenger in diverse functions including vasodilation neurotransmission, anti-tumor and anti-pathogenic activities. NOS is classified under three types: neuronal NOS (nNOS) or brain NOS (bNOS); induciblr NOS (iNOS) or macrophage NOS (mNOS); and endothelial NOS (eNOS). This antibody reacts with iNOS, bNOS and eNOS.

          For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

          Bioinformatics

          Protein Aliases: calcium-calmodulin independent nitric oxide synthase iNOS; cNOS; constitutive endothelial nitric oxide synthase; constitutive NOS; EC-NOS; endothelial nitric oxide synthase; endothelial nitric oxide synthase 3; endothelial NOS; eNOS; hepatocyte NOS; inducible nitric oxide synthase; inducible NO synthase; inducible NOS; macrophage NOS; N-NOS; NC-NOS; neuronal nitric oxide synthase; neuronal NOS; nitric oxidase synthase; nitric oxide synthase; nitric oxide synthase 1 (neuronal); nitric oxide synthase 1, neuronal; nitric oxide synthase 2, inducible; nitric oxide synthase 2, inducible, macrophage; nitric oxide synthase 2A (inducible, hepatocytes); nitric oxide synthase 3 (endothelial cell); nitric oxide synthase 3, endothelial cell; nitric oxide synthase, brain; nitric oxide synthase, endothelial; nitric oxide synthase, inducible-like; nitric oxide synthase, macrophage; nNOS; nNOS mu; NOS type I; NOS type II; NOS type III; NOS, type II; Nos3; NOSII; NOSIII; peptidyl-cysteine S-nitrosylase NOS1; peptidyl-cysteine S-nitrosylase NOS2; unnamed protein product

          View more View less

          Gene Aliases: 2310005C01Rik; 2310065A03Rik; bNOS; cNOS; EC-NOS; ECNOS; eNOS; HEP-NOS; i-NOS; IHPS1; INOS; MAC-NOS; MYMY8; N-NOS; NC-NOS; nNOS; NO; NOS; Nos-1; Nos-2; Nos-3; NOS-I; NOS-II; NOS2A; NOSIII

          View more View less

          UniProt ID: (Human) P29475, (Human) P35228, (Bovine) Q27995, (Human) P29474, (Mouse) Q9Z0J4, (Rat) P29476, (Rat) Q06518, (Mouse) P29477, (Mouse) P70313, (Rat) Q62600

          View more View less

          Entrez Gene ID: (Human) 4842, (Human) 4843, (Bovine) 282876, (Bovine) 287024, (Human) 4846, (Mouse) 18125, (Rat) 24598, (Rat) 24599, (Mouse) 18126, (Mouse) 18127, (Rat) 24600

          View more View less

          Function(s)
          nitric-oxide synthase activity calcium channel regulator activity protein binding calmodulin binding FMN binding oxidoreductase activity sodium channel regulator activity heme binding tetrahydrobiopterin binding arginine binding peptidyl-cysteine S-nitrosylase activity ion channel binding cadmium ion binding calcium-dependent protein binding flavin adenine dinucleotide binding NADP binding scaffold protein binding protein homodimerization activity metal ion binding actin monomer binding superoxide-generating NADPH oxidase activity zinc ion binding enzyme binding identical protein binding ATPase binding phosphoprotein binding RNA polymerase II sequence-specific DNA binding transcription factor binding NADPH binding actin binding beta-catenin binding protein kinase binding cadherin binding nitric-oxide synthase binding Hsp90 protein binding cAMP-dependent protein kinase regulator activity oxidoreductase protein modifying enzyme
          Process(es)
          response to hypoxia regulation of sodium ion transport arginine catabolic process nitric oxide biosynthetic process striated muscle contraction cell communication myoblast fusion blood circulation response to heat response to hormone negative regulation of calcium ion transport into cytosol regulation of cardiac muscle contraction by calcium ion signaling regulation of metal ion transport signaling response to lipopolysaccharide multicellular organismal response to stress nitric oxide-cGMP-mediated signaling pathway xenobiotic catabolic process vasodilation negative regulation of potassium ion transport negative regulation of ion transport positive regulation of neuron apoptotic process cell redox homeostasis negative regulation of blood pressure positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter regulation of neurogenesis negative regulation of serotonin uptake negative regulation of calcium ion transport regulation of cardiac muscle contraction regulation of postsynaptic membrane potential cellular response to growth factor stimulus positive regulation of the force of heart contraction synaptic signaling by nitric oxide positive regulation of adenylate cyclase-activating G protein-coupled receptor signaling pathway positive regulation of sodium ion transmembrane transport regulation of calcium ion transmembrane transport via high voltage-gated calcium channel regulation of blood circulation positive regulation of membrane repolarization during ventricular cardiac muscle cell action potential positive regulation of leukocyte mediated cytotoxicity innate immune response in mucosa superoxide metabolic process inflammatory response circadian rhythm response to bacterium negative regulation of gene expression peptidyl-cysteine S-nitrosylation prostaglandin secretion positive regulation of interleukin-6 production positive regulation of interleukin-8 production Fc-gamma receptor signaling pathway involved in phagocytosis negative regulation of protein catabolic process defense response to bacterium regulation of cellular respiration regulation of insulin secretion defense response to Gram-negative bacterium regulation of cytokine production involved in inflammatory response interleukin-6 production interleukin-8 production oxidation-reduction process mitochondrion organization blood coagulation positive regulation of guanylate cyclase activity cellular response to laminar fluid shear stress negative regulation of extrinsic apoptotic signaling pathway via death domain receptors negative regulation of leukocyte cell-cell adhesion blood vessel remodeling regulation of systemic arterial blood pressure by endothelin aortic valve morphogenesis pulmonary valve morphogenesis endocardial cushion morphogenesis regulation of blood pressure negative regulation of cell proliferation negative regulation of platelet activation positive regulation of gene expression negative regulation of muscle hyperplasia smooth muscle hyperplasia removal of superoxide radicals regulation of neurological system process response to fluid shear stress positive regulation of blood vessel endothelial cell migration endothelial cell migration positive regulation of Notch signaling pathway positive regulation of angiogenesis tetrahydrobiopterin metabolic process nitric oxide metabolic process homeostasis of number of cells within a tissue ventricular septum morphogenesis negative regulation of biomineral tissue development regulation of blood vessel diameter regulation of the force of heart contraction system process negative regulation of iron ion transmembrane transport negative regulation of apoptotic process response to peptide hormone negative regulation of neuron apoptotic process response to estrogen establishment of protein localization positive regulation of G-protein coupled receptor protein signaling pathway negative regulation of vasoconstriction negative regulation of insulin secretion behavioral response to cocaine negative regulation of hydrolase activity negative regulation of cytosolic calcium ion concentration negative regulation of hepatic stellate cell contraction cellular response to mechanical stimulus cellular response to epinephrine stimulus positive regulation of adrenergic receptor signaling pathway positive regulation of long-term synaptic potentiation female pregnancy response to mechanical stimulus response to lead ion response to activity response to nutrient levels response to vitamin E response to vitamin B3 response to nicotine response to decreased oxygen levels regulation of apoptotic process response to ethanol response to nitric oxide endothelial cell proliferation response to glucose response to fructose response to estradiol response to tumor necrosis factor intracellular signal transduction response to immobilization stress regulation of cell proliferation positive regulation of apoptotic process retinoic acid receptor signaling pathway cellular response to lipopolysaccharide cellular response to lithium ion cellular response to vitamin D cellular response to cytokine stimulus cellular response to interferon-gamma cellular response to interleukin-1 cellular response to tumor necrosis factor cellular response to redox state cellular response to xenobiotic stimulus response to dexamethasone response to oxygen-glucose deprivation G-protein coupled receptor signaling pathway coupled to cGMP nucleotide second messenger angiogenesis ovulation from ovarian follicle in utero embryonic development regulation of the force of heart contraction by chemical signal protein import into nucleus gene expression lung development lipopolysaccharide-mediated signaling pathway negative regulation of vascular permeability negative regulation of smooth muscle cell proliferation cellular response to transforming growth factor beta stimulus endothelial tube formation response to xenobiotic stimulus response to carbohydrate response to metal ion cellular response to insulin stimulus response to cytokine cellular response to heat response to erythropoietin response to amino acid response to mercury ion response to axon injury response to corticosterone response to hyperoxia retina development in camera-type eye bone development response to magnetism cellular response to magnesium ion cellular response to cAMP cellular response to high density lipoprotein particle stimulus cellular response to low-density lipoprotein particle stimulus response to peptide response to ketone response to salt response to L-arginine cellular response to rotenone response to ultrasound
          It has to be done as per old AB suggested Products section.

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