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Invitrogen
CYP3A4/CYP3A5 Monoclonal Antibody (F24 P2 B10)
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FIGURE: 1 / 2
CYP3A4/CYP3A5 Antibody (MA3-032) in ICC/IF
Immunofluorescence analysis of Cytochrome P450-3A4-3A5 was done on 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytochrome P450 3A4 + 3A5 (F24 P2 B10) Mouse Monoclonal Antibody (Product # MA3-032) at 1:250 dilution in ... View More
Product Details
MA3-032
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
F24 P2 B10
Immunogen
Synthetic peptide corresponding to residues E S R D G T V S G A, conjugated to ovalbumin.
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-032 detects Cytochrome P450 3A4/3A5 from human cell line HepG2, and from bovine liver and pancreas samples.
MA3-032 has been successfully used in Western blot applications. By Western blot, this antibody detects a ~54 kDa band representing Cytochrome P450 3A4/3A5.
The MA3-032 immunogen is a synthetic peptide corresponding to residues E S R D G T V S G A, conjugated to ovalbumin.
Target Information
CYP3A5 is a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by glucocorticoids and some pharmacological agents. The enzyme metabolizes drugs such as nifedipine and cyclosporine as well as the steroid hormones testosterone, progesterone and androstenedione.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 1,4-cineole 2-exo-monooxygenase; 1,8-cineole 2-exo-monooxygenase; Albendazole monooxygenase (sulfoxide-forming); Albendazole sulfoxidase; aryl hydrocarbon hydroxylase; catalyzes oxidative metabolism of xenobiotics; Cholesterol 25-hydroxylase; CYPIIIA28; CYPIIIA3; CYPIIIA4; CYPIIIA5; cytochrome P-450 nifedipine oxidase; cytochrome P450 3A28; Cytochrome P450 3A3; Cytochrome P450 3A4; Cytochrome P450 3A5; Cytochrome P450 HLp; cytochrome P450 HLp2; Cytochrome P450 NF-25; cytochrome P450, family 3, subfamily A, polypeptide 4; cytochrome P450, family 3, subfamily A, polypeptide 5; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 3; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 4; cytochrome P450, subfamily IIIA (niphedipine oxidase), polypeptide 5; Cytochrome P450-PCN1; Cytochrome P450-PCN3; flavoprotein-linked monooxygenase; glucocorticoid-inducible cytochrome P-450; glucocorticoid-inducible P450; HLp2; microsomal monooxygenase; Nifedipine oxidase; P450-III, steroid inducible; P450-PCN3; Quinine 3-monooxygenase; taurochenodeoxycholate 6-alpha-hydroxylase; unnamed protein product; xenobiotic monooxygenase
Gene Aliases: CP33; CP34; CP35; CYP3A; CYP3A28; CYP3A3; CYP3A4; CYP3A5; CYPIIIA3; CYPIIIA4; CYPIIIA5; HLP; NF-25; P450C3; P450PCN1; P450PCN3; PCN3; VDDR3
UniProt ID: (Human) P08684, (Human) P20815
Entrez Gene ID: (Bovine) 507988, (Human) 1576, (Human) 1577, (Bovine) 282214, (Bovine) 526682
monooxygenase activity
steroid binding
iron ion binding
protein binding
steroid hydroxylase activity
retinoic acid 4-hydroxylase activity
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
oxygen binding
enzyme binding
heme binding
vitamin D3 25-hydroxylase activity
caffeine oxidase activity
metal ion binding
quinine 3-monooxygenase activity
testosterone 6-beta-hydroxylase activity
1-alpha,25-dihydroxyvitamin D3 23-hydroxylase activity
anandamide 8,9 epoxidase activity
anandamide 11,12 epoxidase activity
anandamide 14,15 epoxidase activity
vitamin D 24-hydroxylase activity
estrogen 16-alpha-hydroxylase activity
estrogen 2-hydroxylase activity
1,8-cineole 2-exo-monooxygenase activity
aromatase activity
oxygenase
lipid hydroxylation
lipid metabolic process
fatty acid metabolic process
steroid biosynthetic process
steroid catabolic process
xenobiotic metabolic process
steroid metabolic process
cholesterol metabolic process
androgen metabolic process
estrogen metabolic process
alkaloid catabolic process
monoterpenoid metabolic process
calcitriol biosynthetic process from calciol
xenobiotic catabolic process
vitamin D metabolic process
vitamin D catabolic process
retinol metabolic process
retinoic acid metabolic process
long-chain fatty acid biosynthetic process
aflatoxin metabolic process
oxidative demethylation
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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