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CYP1A2 Monoclonal Antibody (3B8C1)
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FIGURE: 1 / 3
CYP1A2 Antibody (MA3-037) in ICC/IF
Immunofluorescent analysis of Cytochrome P450 1A2 (green) showing staining in the cytoplasm and membrane of COS-7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Cytochrome P450 1A2 monoclonal antibody (Product # MA3-037) in 3% BSA-PBS at a dilution of 1:100 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight-conjugated secondary antibody in PBS ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA3-037
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
3B8C1
Immunogen
Rat cytochrome P450 protein.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-037 detects Cytochrome P450 1A2 from human and non-human primate samples.
MA3-037 has been successfully used in Western blot, immunocytochemistry and immunofluorescence applications. By Western blot, this antibody detects a ~58 kDa band representing Cytochrome P450 1A2.
The MA3-037 immunogen is rat cytochrome P450 protein.
Target Information
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. The protein encoded by this gene localizes to the endoplasmic reticulum and its expression is induced by some polycyclic aromatic hydrocarbons (PAHs), some of which are found in cigarette smoke. The enzyme's endogenous substrate is unknown; however, it is able to metabolize some PAHs to carcinogenic intermediates. Other xenobiotic substrates for this enzyme include caffeine, aflatoxin B1, and acetaminophen. The transcript from this gene contains four Alu sequences flanked by direct repeats in the 3' untranslated region.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: aryl hydrocarbon hydroxylase; cholesterol 25-hydroxylase; CYP; CYPIA2; cytochrome P(3)450; cytochrome P-448; cytochrome P-450 peptide; cytochrome P-450d; cytochrome P450 4; Cytochrome P450 subfamily I (aromatic compound-inducible) member A2 (Q42 form d); cytochrome P450, 1a2; cytochrome P450, family 1, subfamily A, polypeptide 2; cytochrome P450, subfamily I (aromatic compound-inducible), member A2 (Q42, form d); cytochrome P450, subfamily I (aromatic compound-inducible), polypeptide 2; cytochrome P450-D; cytochrome P450-P3; dioxin-inducible P3-450; flavoprotein-linked monooxygenase; hydroperoxy icosatetraenoate dehydratase; microsomal monooxygenase; P(3)450; P-448; P450; P450 4; P450 form 4; P450-D; P450-P3; unnamed protein product; xenobiotic monooxygenase
Gene Aliases: CP12; CYPD45; CYPIA2; P-450d; P3-450; P450(PA); RATCYPD45
UniProt ID: (Human) P05177, (Rat) P04799
Entrez Gene ID: (Human) 1544, (Rat) 24297
monooxygenase activity
iron ion binding
protein binding
steroid hydroxylase activity
arachidonic acid 14,15-epoxygenase activity
electron carrier activity
oxidoreductase activity
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen
oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen
enzyme binding
heme binding
demethylase activity
caffeine oxidase activity
estrogen 16-alpha-hydroxylase activity
estrogen 2-hydroxylase activity
hydroperoxy icosatetraenoate dehydratase activity
fatty acid omega-1 hydroxylase activity
nitrite reductase (NO-forming) activity
steroid catabolic process
porphyrin-containing compound metabolic process
xenobiotic metabolic process
cholesterol metabolic process
estrogen metabolic process
toxin biosynthetic process
toxin metabolic process
response to xenobiotic stimulus
response to bacterium
post-embryonic development
alkaloid metabolic process
regulation of gene expression
monoterpenoid metabolic process
dibenzo-p-dioxin metabolic process
nitroglycerin metabolic process
arachidonic acid metabolic process
lung development
response to estradiol
response to lipopolysaccharide
monocarboxylic acid metabolic process
response to vitamin E
response to immobilization stress
xenobiotic catabolic process
vitamin E metabolic process
retinol metabolic process
cellular respiration
hydrogen peroxide biosynthetic process
oxidative demethylation
cellular response to cadmium ion
cellular response to copper ion
response to tetrachloromethane
response to nitroglycerin
steroid metabolic process
epoxygenase P450 pathway
methylation
primary alcohol metabolic process
hormone metabolic process
long-chain fatty acid biosynthetic process
aflatoxin metabolic process
omega-hydroxylase P450 pathway
olefinic compound metabolic process
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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