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Invitrogen
Acetylcholinesterase Monoclonal Antibody (ZR3)
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FIGURE: 1 / 4
Acetylcholinesterase Antibody (MA3-041) in ICC/IF
Immunofluorescent analysis of Acetylcholinesterase using Acetylcholinesterase Monoclonal antibody (ZR3) (Product # MA3-041) shows staining in U251 glioma cells. Acetylcholinesterase staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an a... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA3-041
Applications
Tested Dilution
Publications
Product Specifications
Host/Isotype
Mouse
/ IgG2b
Class
Monoclonal
Type
Antibody
Clone
ZR3
Immunogen
Purified rat brain acetylcholinesterase.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1.0 mg/mL
Amount
100 µg
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-041 contains 100 µL of in vitro produced, protein A purified IgG in PBS containing 1 mg/mL BSA and 0.05% sodium azide.
MA3-041 detects acetylcholinesterase (AChE) from feline, human, rat, rabbit and guinea pig tissues. This antibody does not cross react with mouse, chicken, bovine, frog, or electric eel AChE.
MA3-041 has been successfully used in immunohistochemistry and immunoprecipitation procedures. This antibody cannot be used in Western blot to detect AChE. Immunohistochemical staining of Ach in rat brain with MA3-041 results in staining of nerve fibers and terminals. MA3-041 reacts with both the soluble and membrane-associated AChE.
The MA3-041 antigen is purified rat brain acetylcholinesterase.
Target Information
Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. The Protein is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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