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TGN46 Monoclonal Antibody (2F7.1)
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FIGURE: 1 / 10
TGN46 Antibody (MA3-063) in ICC/IF
HeLa and COS7 cells were rinsed with PBS and fixed with -20°C methanol for 5 minutes. Cells were incubated with anti-TGN38 antibody (Product # MA3-063) diluted 1:100 in PBS containing 0.2%BSA for 1 hour. The cells were then rinsed in PBS and incubated with goat anti-mouse IgG conjugated to rhodamine for 30 minutes in PBS containing 0.2% BSA. Following washing, cells were imaged using a Zeiss Axioplan microscope. Data courtesy of the Innovators Program.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA3-063
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate,
Rat
Published species
Hamster,
Human,
Mouse,
Non-human primate,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2F7.1
Immunogen
Synthetic peptide corresponding to residues L(18) P S A S K P N N T S S E N N P P(34) C of rat TGN38.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-063 detects trans-Golgi network (TGN) 38 from human and monkey samples and both recombinant and endogenous rat samples.
MA3-063 has been successfully used in Western blot, immunofluorescence, immunocytochemistry and FACS procedures. By Western blot, this antibody detects an ~38 kDa protein representing deglycosylated recombinant rat TGN38. Under normal Western blot conditions, this antibody detects an 85-95 kDa band depending on cell type. Immunofluorescence staining of TGN38 in NRK cells with MA3-063 results in staining predominantly in the TGN.
In immunofluorescence, it has been shown that methanol works best as a fixative.
The MA3-063 immunogen is a synthetic peptide corresponding to residues L(18) P S A S K P N N T S S E N N P P(34) C of rat TGN38.
Target Information
IASPP is one of the most evolutionarily conserved inhibitors of p53 (TP53; MIM 191170), whereas ASPP1. and ASPP2. are activators of p53.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: similar to rat TGN38; TGN38 homolog; TGN46; trans-Golgi network glycoprotein 46; trans-Golgi network glycoprotein 48; trans-Golgi network glycoprotein 51; trans-golgi network protein 1; trans-Golgi network protein TGN51; unnamed protein product
Gene Aliases: hTGN46; hTGN48; hTGN51; TGN38; TGN46; TGN48; TGN51; Tgoln1; Ttgn1; TTGN2
UniProt ID: (Human) O43493, (Rat) P19814
Entrez Gene ID: (Human) 10618, (Rat) 192152
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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