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Invitrogen
FXN Monoclonal Antibody (NPM-1B2)
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FIGURE: 1 / 5
FXN Antibody (MA3-085) in ICC/IF
Immunofluorescent analysis of Frataxin (green) in U2OS cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a Frataxin mouse monoclonal antibody (Product # MA3-085) at a dilution of 1:500 in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG Superclonal™ Secondary Antibody, Alexa Fluor 488 conjugate (Product # A2817... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA3-085
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
NPM-1B2
Immunogen
TrpE-Frataxin full length
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-085 detects Frataxin from human and mouse samples.
MA3-085 has been successfully used in Western blot, immunofluorescence, Flow Cytometry, and ELISA applications.
Target Information
FXN is a mitochondrial protein which belongs to the FRATAXIN family. The protein functions in regulating mitochondrial iron transport and respiration. The expansion of intronic trinucleotide repeat GAA results in Friedreich ataxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Friedreich ataxia; Friedreich ataxia protein; unnamed protein product
Gene Aliases: CyaY; FA; FARR; FRDA; X25
UniProt ID: (Human) Q16595, (Mouse) O35943
Entrez Gene ID: (Human) 2395, (Mouse) 14297
oxidative phosphorylation
heme biosynthetic process
cellular iron ion homeostasis
mitochondrion organization
adult walking behavior
aerobic respiration
embryo development ending in birth or egg hatching
response to iron ion
negative regulation of lipid storage
iron-sulfur cluster assembly
protein autoprocessing
proprioception
cellular cation homeostasis
positive regulation of mitochondrial membrane permeability
negative regulation of multicellular organism growth
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
[2Fe-2S] cluster assembly
[4Fe-4S] cluster assembly
positive regulation of axon extension
negative regulation of organ growth
mitochondrial iron ion transport
positive regulation of lyase activity
regulation of cytosolic calcium ion concentration
protein maturation
cellular response to hydrogen peroxide
negative regulation of release of cytochrome c from mitochondria
inorganic ion homeostasis
mitochondrial respiratory chain complex III assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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