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Invitrogen
SUMO1 Monoclonal Antibody (1SUM-1C6)
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FIGURE: 1 / 2
SUMO1 Antibody (MA3-088) in ICC/IF
Immunofluorescent analysis of SUMO1 (green) in U2OS cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a SUMO1 mouse monoclonal antibody (Product # MA3-088) at a dilution of 1:100 in staining buffer for 1 hour at room temperature, and then incubated with a Goat anti-Mouse IgG Superclonal™ Secondary Antibody, Alexa Fluor 488 conjugate (Product # A28175) at ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA3-088
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
1SUM-1C6
Immunogen
Ovalbumin conjugated synthetic peptide corresponding to aa 85 to aa 97 of human SUMO1.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-088 detects SUMO1 from human and mouse samples. The immunogen sequence is conserved in SUMO1 of mouse origin.
MA3-088 has been successfully used in Western blot, immunofluorescence and ELISA applications.
Ovalbumin conjugated synthetic peptide (C)-EDVIEVYQEQTGG) corresponding to aa 85-97 of human SUMO1.
Target Information
SUMO1 is an ubiquitin-like protein that can be covalently attached to proteins as a monomer or a lysine-linked polymer. Covalent attachment, via an isopeptide bond, to its substrates requires prior activation by the E1 complex SAE1-SAE2 and linkage to the E2 enzyme UBE2I, and can be promoted by E3 ligases such as PIAS1-4, RANBP2 or CBX4. This post-translational modification on lysine residues of proteins plays a crucial role in a number of cellular processes such as nuclear transport, DNA replication and repair, mitosis and signal transduction. SUMO1 is involved, for instance, in targeting RANGAP1 to the nuclear pore complex protein RANBP2. Polymeric SUMO1 chains are also susceptible to polyubiquitination which functions as a signal for proteasomal degradation of modified proteins. SUMO1 may also regulate a network of genes involved in palate development. Mutations in the gene can result in non-syndromic orofacial cleft 10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CG4494-PA; GAP modifying protein 1; LD07775p; Mutations: 288:OK; OTTHUMP00000202403; OTTHUMP00000202404; OTTHUMP00000202405; OTTHUMP00000202406; OTTHUMP00000202409; OTTHUMP00000202410; sentrin; Sentrin-1; similar to ubiquitin and to yeast Smt3p (suppressor of MIF2); Method: conceptual translation supplied by author; SMT3 homolog 3; SMT3 suppressor of mif two 3 homolog 1; SUMO-1 related peptidase; ubiquitin-homology domain protein PIC1; ubiquitin-like 1; ubiquitin-like protein; ubiquitin-like protein SMT3C; ubiquitin-like protein UBL1; UBL1; unnamed protein product
Gene Aliases: DAP1; GMP1; OFC10; PIC1; SENTRIN; SMT3; SMT3C; SMT3H3; SMTP3; SUMO-1; UBL1
UniProt ID: (Human) P63165, (Mouse) P63166
Entrez Gene ID: (Human) 7341, (Mouse) 22218
negative regulation of transcription from RNA polymerase II promoter
DNA repair
protein sumoylation
positive regulation of protein complex assembly
regulation of protein stability
positive regulation of proteasomal ubiquitin-dependent protein catabolic process
cellular response to heat
negative regulation of protein import into nucleus
negative regulation of action potential
negative regulation of transcription, DNA-templated
protein stabilization
palate development
negative regulation of transcription initiation from RNA polymerase II promoter
cellular response to cadmium ion
regulation of cardiac muscle cell contraction
regulation of calcium ion transmembrane transport
regulation of transcription, DNA-templated
PML body organization
protein localization to nuclear pore
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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