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Invitrogen
Phospholamban Monoclonal Antibody (2D12)
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FIGURE: 1 / 70
Phospholamban Antibody (MA3-922) in ICC/IF
Immunofluorescent analysis of Phospholamban (green) showing staining in the cytoplasm of C2C12 cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a Phospholamban monoclonal antibody (Product # MA3-922) in 3% B... View More
Product Details
MA3-922
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Dog,
Chicken,
Guinea pig,
Human,
Mouse,
Sheep,
Pig,
Rabbit,
Rat
Published species
Dog,
Guinea pig,
Human,
Mouse,
Pig,
Rabbit,
Rat,
Rodent
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
2D12
Immunogen
Synthetic peptide corresponding to residues D(2) K V Q Y L T R S A I R R A S T I E M P Q Q A R(25) of canine Phospholamban.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA3-922 detects phospholamban (PLB) in rabbit, human, pig, sheep, chicken, canine, guinea porcine, rat, and mouse.
MA3-922 has been successfully used in Western blot, immunofluorescence, immunohistochemistry and immunoprecipitation procedures. By Western blot, this antibody detects a 25 kDa protein under non-reducing conditions representing the pentameric form of PLB and a 5 kDa protein under reducing conditions representing the monomeric form of PLB in canine cardiac extracts. Immunofluorescence staining of PLB in rat papillary muscle tissue with MA3-922 results in diffuse staining of the network sarcoplasmic reticulum (SR). MA3-922 can also be used to increase the calcium uptake in ventricular SR vesicles analogous to the phosphorylation of PLB following adrenergic stimulation. This antibody is recommended for immunofluorescent staining of tissues.
The MA3-922 immunogen is a synthetic peptide corresponding to residues D(2) K V Q Y L T R S A I R R A S T I E M P Q Q A R(25) of canine PLB. This antibody recognizes an epitope between amino acid residues 9-17 of canine PLB.
Target Information
Phospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cardiac CMH18; cardiac phospholamban; Phospholamban; PLB; unnamed protein product
Gene Aliases: CMD1P; CMH18; PLB; Plm; PLN
UniProt ID: (Chicken) P26677, (Rabbit) P61015, (Pig) P61013, (Dog) P61012, (Human) P26678, (Rat) P61016, (Mouse) P61014
regulation of the force of heart contraction
calcium ion transport
cellular calcium ion homeostasis
regulation of heart contraction
response to zinc ion
negative regulation of heart rate
regulation of release of sequestered calcium ion into cytosol by sarcoplasmic reticulum
regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion
negative regulation of ATPase activity
response to insulin
response to testosterone
negative regulation of heart contraction
cardiac muscle tissue development
protein homooligomerization
regulation of calcium ion transport
negative regulation of calcium ion transport
regulation of ryanodine-sensitive calcium-release channel activity
regulation of cardiac muscle cell contraction
adrenergic receptor signaling pathway involved in heart process
regulation of the force of heart contraction by cardiac conduction
regulation of calcium ion import
negative regulation of calcium ion transmembrane transporter activity
regulation of relaxation of muscle
negative regulation of calcium ion binding
regulation of calcium-transporting ATPase activity
negative regulation of calcium-transporting ATPase activity
negative regulation of catalytic activity
Notch signaling pathway
negative regulation of calcium ion import into sarcoplasmic reticulum
acrosome assembly
spermatogenesis
blood circulation
visual learning
locomotor rhythm
muscle cell cellular homeostasis
circadian sleep/wake cycle, sleep
regulation of cytosolic calcium ion concentration
relaxation of cardiac muscle
regulation of cardiac muscle cell membrane potential
negative regulation of calcium ion import
regulation of relaxation of cardiac muscle
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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