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Invitrogen
beta Tubulin Monoclonal Antibody (TBN06 (Tub 2.5))
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FIGURE: 1 / 11
beta Tubulin Antibody (MA5-11732) in ICC/IF
Immunofluorescent analysis of beta-tubulin (green) in COS7 and NRK cells. Cells were fixed and permeabilized with ice-cold methanol for 10 minutes at room temperature, and blocked with 0.3% BSA in PBS for at least 15 minutes at room temperature. Cells were probed with a beta-tubulin monoclonal antibody (Product # MA5-11732) at a dilution of 1:50 overnight at 4°C. Cells were washed with PBS, and incubated wi... View More
Product Details
MA5-11732
Applications
Tested Dilution
Publications
Product Specifications
Published species
Cat,
Human,
Mouse,
Plant,
Rat,
Zebrafish
Host/Isotype
Mouse
/ IgG3
Class
Monoclonal
Type
Antibody
Clone
TBN06 (Tub 2.5)
Immunogen
Purified tubulin
Conjugate
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Storage conditions
4°C
Shipping conditions
Wet ice
RRID
Product Specific Information
MA5-11732 targets Tubulin Beta in IF, IHC (P), and WB applications and shows reactivity with Amphibian, Bovine, Chicken, Human, Insect, mouse, Plant, Rabbit, and Rat samples.
Target Information
Beta tubulins are one of two core protein families (alpha and beta tubulins) that heterodimerize and assemble to form microtubules. Beta-III tubulin is primarily expressed in neurons and may be involved in neurogenesis, axon guidance and maintenance. Mutations in the beta tubulin gene are the cause of congenital fibrosis of the extraocular muscles type 3. Beta-III tubulin was also detected in Sertoli cells of the testis and transiently in non-neuronal embryonic tissues. Glutamate residues at the C-terminus of beta III tubulin can be glutamylated. The precise function of such modifications is unclear. Tubulin is phosphorylated on Ser-172 by CDK1 during cell cycle progression. Ser-172 phosphorylation inhibits tubulin incorporation into microtubules.Microtubules, the major cytoskeletal elements found in all eukaryotic cells, consist of Tubulin, which is a dimer of two 55 kDa subunits: alpha and Beta. Microtubules play key roles in chromosome segregation in mitosis, intracellular transport, ciliary and flagellar bending, and structural support of the cytoskeleton. Because beta-tubulin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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