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Invitrogen
CD24 Monoclonal Antibody (SN3b)
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FIGURE: 1 / 14
CD24 Antibody (MA5-11833) in ICC/IF
Immunofluorescent analysis of CD24 (green) showing staining in the membrane and cytoplasm of Ramos cells (right) compared to a negative control without primary antibody (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD24 monoclonal antibody (Product # MA5-11833) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4°C in a humidified chamber. Cells were washed with PBST and incubated with a DyLi... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-11833
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Host/Isotype
Mouse
/ IgM, kappa
Class
Monoclonal
Type
Antibody
Clone
SN3b
Immunogen
Cell membrane antigen preparation isolated from NALM-1 human pre-B leukemia cell line
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.8 mg/mL
Amount
400 µg
Storage conditions
4°C
Shipping conditions
Wet ice
RRID
Product Specific Information
MA5-11833 targets CD24 in WB, ICC/IF, FACS and IHC (P) applications and shows reactivity with Human and Mouse samples. This antibody is not suitable for mouse BAF-3 cell lysates in Western blot analysis.
The MA5-11833 immunogen is cell membrane antigen preparation isolated from NALM-1 human pre-B leukemia cell line.
Target Information
CD24 is a 35-50 kDa glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed on a variety of cell types, including erythrocytes, thymocytes, peripheral lymphocytes, and cells of the myeloid lineage. It is variably glycosylated, resulting in heterogeneity in molecular mass across different cell lineages, which can affect antibody staining levels on lymphocyte populations. In the context of B-cell development, CD24 is expressed from the pro-B-cell stage in the bone marrow through to mature, surface Ig-positive B cells, with very low or negative expression on plasma cells. It is also present on the majority of B-lineage acute lymphoblastic leukemias, B-cell chronic lymphocytic leukemias (CCLs), and B-cell non-Hodgkin's lymphomas. CD24 is thought to play a role in regulating B-cell proliferation and maturation, as well as in controlling autoimmunity. Additionally, CD24 has been reported to interact with P-selectin (CD62P), indicating a potential role in cell adhesion and signaling. CD24 is a valuable marker for studying B-cell development, leukemia, lymphoma, and autoimmune regulation, as well as its interactions with other molecules like P-selectin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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