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Invitrogen
TIMP2 Monoclonal Antibody (T2-101)
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FIGURE: 1 / 5
TIMP2 Antibody (MA5-13603) in WB
Western blot was performed using Anti-TIMP2 Monoclonal Antibody (T2-101) (Product # MA5-13603) and a 24kDa band corresponding to TIMP2 was observed in HT-1080 but was absent in Daudi which is reported to be negative. Whole cell extracts (30 µg lysate) of HT-1080 (Lane 1), HT-1080 treated with Protein Transport Inhibitor (PTI; 1x for 4 hours) (Lane 2), Daudi (Lane 3) and Daudi treated with Protein Transport Inhibitor (1x for 4 hours) (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved prote... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-13603
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
T2-101
Immunogen
Native human pro-MMP-2/TIMP-2 complex purified from A2058 culture medium followed by purified human rTIMP-2
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-13603 targets TIMP-2 in Immunoprecipitation and Western blot applications and shows reactivity with Human samples.
The MA5-13603 immunogen is native human pro-MMP-2/TIMP-2 complex purified from A2058 culture medium followed by purified human rTIMP-2.
Target Information
TIMP-1 and TIMP-2 are inhibitory enzymes of matrix metalloproteinase family, and are thought to be of great importance in the maintenance of connective tissue integrity. TIMP-2 shows the highest binding affinity to both the latent (pro) and active forms of 72 kDa Type IV collagenase (also known as MMP-2 or gelatinase A). It also has affinity for the active form of 92 kDa Type IV collagenase (also known as MMP-9 or gelatinase B). TIMP-2 inhibits the proteolytic invasiveness of tumor cells and normal placental trophoblast cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: metalloproteinase inhibitor precursor; metalloproteinase-2 inhibitor precursor; testicular secretory protein Li 57; TIMP 2; tissue inhibitor of metalloproteinases 2; unnamed protein product
Gene Aliases: CSC-21K; D11Bwg1104e; DDC8; Timp-2
UniProt ID: (Human) P16035
Entrez Gene ID: (Human) 7077, (Mouse) 21858
response to hormone
response to cytokine
negative regulation of proteolysis
negative regulation of membrane protein ectodomain proteolysis
negative regulation of metallopeptidase activity
negative regulation of cell proliferation
regulation of Rap protein signal transduction
regulation of MAPK cascade
positive regulation of MAPK cascade
regulation of neuron differentiation
positive regulation of neuron differentiation
positive regulation of adenylate cyclase activity
negative regulation of mitotic cell cycle
negative regulation of Ras protein signal transduction
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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