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Invitrogen
POLB Monoclonal Antibody (18S)
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-13899
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Bovine,
Hamster,
Human,
Mouse,
Rat,
Xenopus
Published species
Human,
Mouse,
Rabbit,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
18S
Immunogen
Rat DNA polymerase beta protein
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS, pH 7.4, with 0.2% BSA
Contains
0.09% sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-13899 targets Rat DNA Polymerase beta in IP and WB applications and shows reactivity with Bovine, Hamster, Human, mouse, Rat, and Xenopus laevis samples.
The MA5-13899 immunogen is rat DNA polymerase beta protein.
Target Information
DNA polymerase beta comprises an amino-terminal 8- kDa domain and a carboxy-terminal 31- kDa domain. The N-terminal ssDNA binding domain has a deoxyribose phosphodiesterase activity while the C-terminal domain has a nucleotidyltransferase activity. Mammalian DNA polymerase beta, a DNA repair polymerase, is constitutively expressed in cultured cells, but treatment of cells with the DNA-alkylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) or methyl methanesulfonate up-regulates beta-pol level. DNA polymerase beta fills single nucleotide gaps in DNA produced by the base excision repair pathway of mammalian cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 5'-deoxyribose-phosphate lyase; 5'-dRP lyase; AP lyase; DNA pol beta; DNA polymerase beta; DNA polymerase beta subunit; DNA-directed DNA polymerase beta; MGC125976; POL B; Pol beta; POLB; polymerase (DNA directed), beta; polymerase (DNA) beta; polymerase B; polymerase beta; polymerase-beta; unnamed protein product
Gene Aliases: A430088C08Rik; polb; XELAEV_18020165mg
UniProt ID: (Human) P06746, (Bovine) Q27958, (Mouse) Q8K409, (Rat) P06766, (Xenopus) O57383
Entrez Gene ID: (Human) 5423, (Bovine) 614688, (Mouse) 18970, (Chinese hamster) 100774965, (Rat) 29240, (Xenopus) 397973
DNA binding
damaged DNA binding
DNA-directed DNA polymerase activity
DNA-(apurinic or apyrimidinic site) lyase activity
protein binding
microtubule binding
nucleotidyltransferase activity
lyase activity
enzyme binding
DNA polymerase activity
metal ion binding
5'-deoxyribose-5-phosphate lyase activity
class I DNA-(apurinic or apyrimidinic site) endonuclease activity
DNA-directed DNA polymerase
DNA-dependent DNA replication
DNA repair
regulation of DNA repair
base-excision repair
base-excision repair, gap-filling
pyrimidine dimer repair
nucleotide-excision repair, DNA gap filling
double-strand break repair via nonhomologous end joining
cellular response to DNA damage stimulus
response to gamma radiation
response to ethanol
response to hyperoxia
DNA biosynthetic process
in utero embryonic development
apoptotic process
inflammatory response
salivary gland morphogenesis
intrinsic apoptotic signaling pathway in response to DNA damage
somatic diversification of immunoglobulins
somatic hypermutation of immunoglobulin genes
lymph node development
spleen development
homeostasis of number of cells
neuron apoptotic process
immunoglobulin heavy chain V-D-J recombination
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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