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Invitrogen
beta Catenin Monoclonal Antibody (E247)
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FIGURE: 1 / 8
beta Catenin Antibody (MA5-14461) in ICC/IF
Immunofluorescence analysis of beta Catenin was performed using 70% confluent log phase A431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with beta Catenin (E247) Rabbit monoclonal antibody (Product # MA5-14461) at 1:250 in 0.1% BSA and incubated for 3 ... View More
Product Details
MA5-14461
Applications
Tested Dilution
Publications
Product Specifications
Published species
Human
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
E247
Immunogen
A synthetic peptide derived from near the N-terminus of human beta-catenin
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage conditions
4°C
Shipping conditions
Wet ice
RRID
Product Specific Information
MA5-14461 targets beta-Catenin in IHC (P) applications and shows reactivity with Human samples.
The MA5-14461 immunogen is a synthetic peptide derived from near the N-terminus of human beta-catenin.
Target Information
Beta-catenin, an adherens junction (AJ) protein, was originally identified as a component of cell-cell adhesion structures. AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. Beta-catenin interacts with the cytoplasmic domain of E-cadherin and links E-cadherin to alpha-catenin, which in turn mediates anchorage of the E-cadherin complex to the cortical actin cytoskeleton. Studies show that Beta-catenin also binds to another cytoskeletal complex containing the adenomatous polyposis coli protein and microtubules, and interacts with several signaling pathways that include tyrosine kinases, phosphatases and Wnt/Wingless. The interplay between beta-catenin, cytoskeletal complexes and signaling pathways may regulate morphogenesis. Beta-catenin is expressed in several hair follicle cell types, basal and peripheral matrix cells, and cells of the outer and inner root sheats. A pathological role of beta-catenin has been identified in pilomatrixoma (PTR), medulloblastoma (MDB), colorectal cancer (CRC), ovarian cancer, and tumor development. In the nucleus, beta-catenin serves to co activate a family of Lef/Tcf transcription factors that stimulate transcription of target genes including those encoding cyclin D and c-myc that promote cell proliferation. The influence on cell proliferation is the molecular basis for the role of beta-catenin in tumorgenesis, specifically, solid tumors of the breast, colon, liver, lung, gastric, prostate, and skin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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