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Invitrogen
alpha-1 Antitrypsin Monoclonal Antibody (TMF1#4B5)
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FIGURE: 1 / 5
alpha-1 Antitrypsin Antibody (MA5-14661) in WB
Western blot analysis was performed on membrane enriched extracts (30 µg) of (Fig 1) HT-29 (Lane 1) and Hep G2 (Lane 2).Likewise, Western blot analysis was performed on (Fig 2) condition media of Hep G2 (Lane 1), PC3 (Lane 2) and HeLa (Lane 3).The blots were probed with Anti-Alpha1-Antitrypsin Mouse Monoclonal Antibody (Product # MA5-14661, 2 µg/mL) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 µg/mL, 1:2500 dilution). In Figure 1, a ~47 kDa band corr... View More
Product Details
MA5-14661
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Host/Isotype
Mouse
/ IgG2a
Class
Monoclonal
Type
Antibody
Clone
TMF1#4B5
Immunogen
Purified human a1-Antitrypsin
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.4, with 1mg/mL BSA
Contains
0.05% sodium azide
Storage conditions
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-14661 targets Alpha1-Antitrypsin in ELISA, IP, and RIA applications and shows reactivity with Human samples.
The MA5-14661 immunogen is purified human a1-Antitrypsin.
MA5-14661 detects Alpha1-Antitrypsin which has a predicted molecular weight of approximately 46 kDa.
Product MA514661 is a smaller package size of MIA1101 (formerly sold as a Seradyn product).
Target Information
Alpha-1-AT is synthesized in the liver and it acts as an inhibitor of proteases such as trypsin, elastase, chymotrypsin, collagenase, leucocytic proteases, plasmin, and thrombin, which may be released during inflammatory reactions in the lung. In the absence of alpha-1-AT, these enzymes are not inhibited and they may digest pulmonary parenchyma. Alpha-1-AT deficiency is associated with chronic obstructive lung disease (emphysema) and less frequently with hepatic cirrhosis in infants and respiratory distress of the newborn. Increase in alpha-1-AT occurs as an acute phase response to tissue necrosis and inflammation. Serum level of alpha-1-AT is elevated in rheumatoid arthritis, bacterial infections, vasculitis, and carcinomatosis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: a-1 AT; a1-antitrypsin; AAT; alpha 1 antitrypsin; alpha-1 antitrypsin; Alpha-1 protease inhibitor; Alpha-1 protease inhibitor 1; alpha-1 proteinase inhibitor; Alpha-1-antiproteinase; Alpha-1-antitrypsin; alpha-1-antitrypsin (alpha-1-AT); Alpha-1-antitrypsin 1-1; alpha-1-antitrypsin 2; anti-elastase; Digestive Zymogen; epididymis secretory sperm binding protein; MGC23330; MGC9222; protease inhibitor; protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin; Protease Serine 1; proteolytic enzyme inhibitor; serine (or cysteine) proteinase inhibitor, clade A, member 1; serine (or cysteine) proteinase inhibitor, clade A, member 1a; Serine Protease 1; Serine protease inhibitor 1-1; Serine protease inhibitor A1a; Serpin A1; Serpin A1a; serpin peptidase inhibitor clade A (alpha-1 antiproteinase antitrypsin) member 1 isoform SERPINA1_1; serpin peptidase inhibitor clade A (alpha-1 antiproteinase antitrypsin) member 1 isoform SERPINA1_2; serpin peptidase inhibitor clade A (alpha-1antiproteinase, antitrypsin) member 1; serpin peptidase inhibitor clade A member 1; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1; TRP1; TRY1; unnamed protein product
Gene Aliases: A1A; A1AT; AAT; Aat-2; Aat2; alpha1AT; Dom1; nNIF; PI; PI1; PRO0684; PRO2209; PRO2275; SERPINA1; Serpina1a; Spi1-1; Spi1-3
UniProt ID: (Human) P01009, (Mouse) P07758
Entrez Gene ID: (Human) 5265, (Mouse) 20700
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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