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Invitrogen
SIRT3 Monoclonal Antibody (K.830.1)
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FIGURE: 1 / 6
SIRT3 Antibody (MA5-14910) in ICC/IF
Immunofluorescence analysis of NAD-dependent protein deacetylase sirtuin-3, mitochondrial was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with SIRT3 Monoclonal Antibody (K.830.1) (Product # MA5-14910) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (H+L) Highly Cross-Adso... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-14910
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
K.830.1
Immunogen
Synthetic peptide corresponding to the sequence of human SirT3 protein
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
73 µg/mL
Amount
7.3 µg
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 50% glycerol, 0.15M NaCl, 100µg/mL BSA
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with other sirtuins.
Target Information
SIRT3 is a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The SIRT3 is included in class I of the sirtuin family.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: HGNC:14931; mitochondrial nicotinamide adenine dinucleotide-dependent deacetylase; NAD-dependent deacetylase sirtuin-3, mitochondrial; NAD-dependent deacetylase; sirtuin 3 long reading frame; NAD-dependent protein delactylase sirtuin-3; regulatory protein SIR2 homolog 3; silent mating type information regulation 2, S.cerevisiae, homolog 3; sir2-like 3; SIR2-like protein 3; SIRT3L mitochondrial; sirtuin type 3; unnamed protein product; yeast silent information regulator 2 (Sir2p) homolog
Gene Aliases: SIR2L3
UniProt ID: (Human) Q9NTG7
Entrez Gene ID: (Human) 23410, (Rat) 293615
NAD+ ADP-ribosyltransferase activity
protein binding
zinc ion binding
transferase activity, transferring acyl groups other than amino-acyl groups
NAD-dependent histone deacetylase activity
enzyme binding
protein deacetylase activity
NAD-dependent protein deacetylase activity
sequence-specific DNA binding
NAD+ binding
NAD-dependent protein lysine delactylase activity
protein ADP-ribosylase activity
NAD-dependent histone deacetylase activity (H3-K14 specific)
chromatin remodeling
protein deacetylation
mitochondrial fusion
aerobic respiration
positive regulation of insulin secretion
cellular response to stress
peptidyl-lysine deacetylation
negative regulation of ERK1 and ERK2 cascade
positive regulation of superoxide dismutase activity
positive regulation of catalase activity
positive regulation of oxidative phosphorylation
positive regulation of ceramide biosynthetic process
negative regulation of reactive oxygen species metabolic process
positive regulation of transcription from mitochondrial promoter
negative regulation of peptidyl-lysine acetylation
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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