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Invitrogen
c-Cbl Monoclonal Antibody (S.551.7)
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FIGURE: 1 / 4
c-Cbl Antibody (MA5-14955) in WB
Western blot was performed using Anti-c-Cbl Monoclonal Antibody (S.551.7) (Product # MA5-14955) and a 110 kDa band corresponding to c-Cbl was observed. Whole cell extracts (30 µg lysate) of THP-1 (Lane 1), BeWo (Lane 2), Daudi (Lane 3) and SH-SY5Y (Lane 4) were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilut... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-14955
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
S.551.7
Immunogen
Synthetic peptide corresponding to the sequence of human c-Cbl
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
18.6 µg/mL
Amount
1.86 µg
Purification
Affinity chromatography
Storage buffer
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
Contains
<0.02% sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with Cbl-b or Cbl-c proteins.
Target Information
Cbl proteins are a family of ubiquitin protein ligases (E3s) that negatively regulate signaling by targeting activated tyrosine kinases for degradation. Cbl- c is the most recently cloned member of the Cbl proteins and is expressed only in epithelial cells (the other Cbl proteins are ubiquitously expressed). Cbl-c, like the other mammalian Cbl proteins, can ubiquitinate the activated EGFR and target it for degradation. Through interactions with proteins containing SRC homology-2 (SH2) and SH3 domains, CBL proteins modulate downstream cell signaling.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: c-cbl; Cas-Br-M (murine) ecotropic retroviral transforming sequence; casitas B-lineage lymphoma proto-oncogene; Cbl proto-oncogene, E3 ubiquitin protein ligase; CBL-3; CBLC; fragile site, folic acid type, rare, fra(11)(q23.3); oncogene CBL2; proto-oncogene c-Cbl; RING finger protein 55; RING-type E3 ubiquitin transferase CBL; RNF57; signal transduction protein CBL
Gene Aliases: C-CBL; CBL2; FRA11B; NSLL; RNF55
UniProt ID: (Human) P22681
Entrez Gene ID: (Human) 867
phosphotyrosine binding
ubiquitin-protein transferase activity
calcium ion binding
protein binding
SH3 domain binding
protein kinase binding
receptor tyrosine kinase binding
phosphatidylinositol 3-kinase regulatory subunit binding
cadherin binding
metal ion binding
ephrin receptor binding
ubiquitin protein ligase activity
protein tyrosine kinase binding
protein polyubiquitination
ubiquitin-dependent protein catabolic process
protein monoubiquitination
cellular response to DNA damage stimulus
signal transduction
cell surface receptor signaling pathway
male gonad development
response to gamma radiation
response to activity
protein ubiquitination
cytokine-mediated signaling pathway
regulation of signaling
response to testosterone
cellular response to platelet-derived growth factor stimulus
negative regulation of epidermal growth factor receptor signaling pathway
response to starvation
negative regulation of apoptotic process
mast cell degranulation
response to ethanol
positive regulation of epidermal growth factor receptor signaling pathway
viral entry into host cell
positive regulation of receptor-mediated endocytosis
protein stabilization
negative regulation of T cell receptor signaling pathway
negative regulation of T cell activation
protein autoubiquitination
positive regulation of protein kinase B signaling
ubiquitin-dependent endocytosis
protein K63-linked ubiquitination
cellular response to hypoxia
cellular response to nerve growth factor stimulus
regulation of platelet-derived growth factor receptor-alpha signaling pathway
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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