Search
Invitrogen
Neurofilaments 70/200 kDa Monoclonal Antibody (2F11)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
FIGURE: 1 / 6
Neurofilaments 70/200 kDa Antibody (MA5-15234) in ICC/IF
Immunofluorescence analysis of CD79a was performed using 70% confluent log phase SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CD79a (2F11) Mouse Monoclonal Antibody (Product # MA5-15234) at 1:250 dilution in 0.1% BSA and incubated for 3 hou... View More
Product Details
MA5-15234
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Cat,
Human,
Mouse,
Rabbit,
Rat
Published species
Avian,
Fish,
Mouse,
Rabbit
Host/Isotype
Mouse
/ IgG1, kappa
Class
Monoclonal
Type
Antibody
Clone
2F11
Immunogen
Neurofilament purified from human brain
Conjugate
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
tissue culture supernatant
Contains
15mM sodium azide
Storage conditions
4°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-15234 targets Neurofilament (200kDa and 68kDa) in IF, IHC (P), IP, and WB applications and shows reactivity with Feline, Human, mouse, Rabbit, and Rat samples.
The MA5-15234 immunogen is neurofilament purified from human brain.
Target Information
Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. NEFH has an important function in mature axons that is not subserved by the two smaller NF proteins. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: 200 kDa neurofilament protein; 68 kDa neurofilament protein; hSTEp; intermediate filament; light molecular weight neurofilament protein; NEFH/NEFL; NEFL/NEFH; neurofilament 200kDa; Neurofilament heavy polypeptide; Neurofilament light polypeptide; neurofilament protein L; neurofilament protein, light chain; neurofilament subunit NF-L; Neurofilament triplet H protein; Neurofilament triplet L protein; neurofilament, heavy polypeptide 200kDa; neurofilament, light polypeptide 68kDa; neurofilament-H; NF-H; NF-L; protein phosphatase 1, regulatory subunit 110; unnamed protein product
Gene Aliases: AI847934; CMT1F; CMT2CC; CMT2E; CMTDIG; KIAA0845; mKIAA0845; NEFH; NEFL; NF-H; NF-L; NF200; NF68; NFH; NFL; PPP1R110
UniProt ID: (Human) P12036, (Human) P07196, (Mouse) P19246, (Mouse) P08551, (Rat) P19527
Entrez Gene ID: (Human) 4744, (Rabbit) 100357177, (Human) 4747, (Rabbit) 100353082, (Rat) 24587, (Mouse) 380684, (Mouse) 18039, (Rat) 83613
structural constituent of cytoskeleton
protein binding
microtubule binding
kinesin binding
protein kinase binding
protein binding, bridging
dynein complex binding
structural constituent of postsynaptic intermediate filament cytoskeleton
tubulin binding
protein domain specific binding
identical protein binding
phospholipase binding
macromolecular complex binding
structural molecule activity
toxic substance binding
nutrient reservoir activity
protein heterodimerization activity
protein C-terminus binding
microtubule cytoskeleton organization
axonogenesis
anterograde axonal transport
retrograde axonal transport
response to toxic substance
peripheral nervous system axon regeneration
axonal transport of mitochondrion
spinal cord development
hippocampus development
cerebral cortex development
regulation of axon diameter
neurofilament bundle assembly
locomotion
response to peptide hormone
intermediate filament polymerization or depolymerization
intermediate filament organization
intermediate filament bundle assembly
neuron projection morphogenesis
positive regulation of axonogenesis
neuromuscular process controlling balance
protein polymerization
response to corticosterone
neurofilament cytoskeleton organization
regulation of synapse maturation
motor neuron apoptotic process
postsynaptic modulation of chemical synaptic transmission
postsynaptic intermediate filament cytoskeleton organization
response to sodium arsenite
response to acrylamide
negative regulation of motor neuron apoptotic process
cell projection assembly
intermediate filament cytoskeleton organization
peripheral nervous system neuron axonogenesis
axon development
regulation of organelle transport along microtubule
cellular response to leukemia inhibitory factor
negative regulation of neuron apoptotic process
ovarian follicle atresia
brain development
embryo development
axon regeneration
neuron projection development
cellular response to oxidative stress
response to cocaine
cellular response to estradiol stimulus
response to organic substance
response to organonitrogen compound
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support