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Invitrogen
IKK beta Monoclonal Antibody (10A2C5B3)
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FIGURE: 1 / 2
IKK beta Antibody (MA5-15320) in IHC (P)
Immunohistochemical analysis of paraffin-embedded human colon carcinoma (A), breast carcinoma (B), kidney cell carcinoma (C), bladder carcinoma tumor (D), showing membrane and cytoplasmic localization using IKBKB monoclonal antibody (Product # MA5-15320) followed with DAB staining.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-15320
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
10A2C5B3
Immunogen
Purified recombinant fragment of IKBKB expressed in E. Coli.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.03% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-15320 targets IKBKB in indirect ELISA, IHC and WB applications and shows reactivity with Human samples.
The MA5-15320 immunogen is purified recombinant fragment of IKBKB expressed in E. Coli.
Target Information
Phosphorylation of serine residues on the I-kappa-B proteins by IKK kinases, including IKBKB, marks them for destruction via the ubiquitination pathway, allowing activation of the NF-kappa-B complex. Inhibitor of nuclear factor kappa-B kinase subunit beta (IKK beta) is a serine kinase that plays an essential role in the NF-kaapa-B signaling pathway which is activated by mulitple stimuli such as inflammatory cytokines, bacterial, or viral products, DNA damages, or other cellular stresses. IKK beta acts as part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues. These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome. In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis. Mutations affecting the IKK beta gene can result in Immunodeficiency 15 (IMD15).
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: FLJ40509; I-kappa-B kinase 2; I-kappa-B-kinase beta; inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta; MGC131801; nuclear factor kappa B kinase subunit beta; nuclear factor NF-kappa-B inhibitor kinase beta; serine/threonine protein kinase IKBKB; unnamed protein product
Gene Aliases: IKK-2; IKK-beta; IKK2; IKKB; IMD15; IMD15A; IMD15B; NFKBIKB
UniProt ID: (Human) O14920
Entrez Gene ID: (Human) 3551
protein kinase activity
protein serine/threonine kinase activity
protein binding
ATP binding
IkappaB kinase activity
protein kinase binding
identical protein binding
protein homodimerization activity
protein heterodimerization activity
scaffold protein binding
protein serine kinase activity
transferrin receptor binding
pattern recognition receptor signaling pathway
stimulatory C-type lectin receptor signaling pathway
antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent
MyD88-dependent toll-like receptor signaling pathway
protein phosphorylation
inflammatory response
I-kappaB kinase/NF-kappaB signaling
response to virus
regulation of tumor necrosis factor-mediated signaling pathway
peptidyl-serine phosphorylation
tumor necrosis factor-mediated signaling pathway
regulation of toll-like receptor signaling pathway
toll-like receptor 3 signaling pathway
TRIF-dependent toll-like receptor signaling pathway
Fc-epsilon receptor signaling pathway
positive regulation of I-kappaB kinase/NF-kappaB signaling
innate immune response
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
T cell receptor signaling pathway
stress-activated MAPK cascade
protein maturation
interleukin-1-mediated signaling pathway
cellular response to tumor necrosis factor
protein localization to plasma membrane
negative regulation of cytokine production involved in inflammatory response
regulation of establishment of endothelial barrier
negative regulation of bicellular tight junction assembly
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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