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Invitrogen
GATA3 Monoclonal Antibody (1A10D1)
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-15387
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Published species
Human
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
1A10D1
Immunogen
Purified recombinant fragment of GATA3 (aa175-388) expressed in E. Coli.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.03% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-15387 targets GATA3 in indirect ELISA, WB applications and shows reactivity with Human samples.
The MA5-15387 immunogen is purified recombinant fragment of GATA3 (aa175-388) expressed in E. Coli.
Target Information
The genes for all 4 subunits of the T-cell antigen receptor (alpha, beta, gamma and delta) are controlled by distinct enhancers and their enhancer-binding proteins. Marine and Winoto (1991) identified a common TCR regulatory element by demonstrating binding of the enhancer-binding protein GATA3 to the enhancer elements of all 4 TCR genes. GATA3 had been shown in the chicken to be an enhancer-binding protein containing a zinc finger domain. GATA3 mRNA was demonstrated by Northern blot analysis in T cells but not in B cells or macrophages. GATA3 is abundantly expressed in the T-lymphocyte lineage and is thought to participate in T-cell receptor gene activation through binding to enhancers. Labastie et al. (1994) cloned the human gene and the 5-prime end of the mouse gene. The human gene comprises 6 exons distributed over 17 kb of DNA. Its 2 zinc fingers are encoded by 2 separate exons highly conserved with those of GATA1, but no other structural homologies between the 2 genes could be found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: GATA binding protein-3; GATA-3; GATA-binding factor 3; MGC2346; MGC5199; MGC5445; Transacting T-cell-specific transcription factor GATA-3; unnamed protein product
Gene Aliases: HDR; HDRS
UniProt ID: (Human) P23771
Entrez Gene ID: (Human) 2625
transcription regulatory region sequence-specific DNA binding
RNA polymerase II regulatory region sequence-specific DNA binding
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
core promoter proximal region sequence-specific DNA binding
transcription coactivator binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
transcriptional activator activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
interleukin-2 receptor binding
protein binding
zinc ion binding
identical protein binding
sequence-specific DNA binding
RNA polymerase II sequence-specific DNA binding transcription factor binding
E-box binding
HMG box domain binding
histone methyltransferase binding
sequence-specific double-stranded DNA binding
negative regulation of transcription from RNA polymerase II promoter
cell fate determination
type IV hypersensitivity
regulation of cytokine production
kidney development
mesonephros development
immune system development
aortic valve morphogenesis
cardiac right ventricle morphogenesis
ventricular septum development
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
defense response
inflammatory response
signal transduction
negative regulation of cell proliferation
male gonad development
response to xenobiotic stimulus
response to virus
anatomical structure morphogenesis
positive regulation of signal transduction
response to gamma radiation
gene expression
positive regulation of endothelial cell migration
negative regulation of epithelial to mesenchymal transition
cell differentiation
T cell differentiation
macrophage differentiation
regulation of epithelial cell differentiation
TOR signaling
positive regulation of interleukin-13 production
positive regulation of interleukin-4 production
positive regulation of interleukin-5 production
negative regulation of mammary gland epithelial cell proliferation
cellular response to interferon-alpha
norepinephrine biosynthetic process
protein kinase B signaling
ear development
response to estrogen
T-helper 2 cell differentiation
cell fate commitment
response to ethanol
positive regulation of T cell differentiation
negative regulation of cell cycle
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
sympathetic nervous system development
embryonic organ development
anatomical structure formation involved in morphogenesis
system development
negative regulation of inflammatory response
regulation of developmental process
T cell receptor signaling pathway
cartilage development
positive regulation of protein kinase B signaling
pharyngeal system development
uterus development
canonical Wnt signaling pathway
mesenchymal to epithelial transition
ureteric bud formation
cellular response to interleukin-4
cellular response to tumor necrosis factor
positive regulation of ureteric bud formation
nephric duct morphogenesis
nephric duct formation
regulation of nephron tubule epithelial cell differentiation
lymphocyte migration
cochlea development
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
negative regulation of cell motility
negative regulation of endothelial cell apoptotic process
positive regulation of thyroid hormone generation
positive regulation of transcription regulatory region DNA binding
regulation of cellular response to X-ray
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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