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Invitrogen

WNT1 Monoclonal Antibody (10C8)

1 Published Figure
2 References
View all (13) WNT1 antibodies

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Product Details

MA5-15544

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:2,000
View 1 publication 1 publication

Immunohistochemistry (IHC)

-
View 1 publication 1 publication

Immunohistochemistry (Paraffin) (IHC (P))

1:200-1:1,000
-

Immunocytochemistry (ICC/IF)

1:200-1:1,000
-

Flow Cytometry (Flow)

1:200-1:400
-

ELISA (ELISA)

1:10,000
-
Product Specifications

Species Reactivity

Human, Mouse

Published species

Human, Rat

Host/Isotype

Mouse / IgG1

Class

Monoclonal

Type

Antibody

Clone

10C8

Immunogen

Purified recombinant fragment of WNT1 expressed in E. Coli.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

Conc. Not Determined

Storage buffer

ascites

Contains

0.03% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10984682

Product Specific Information

MA5-15544 targets WNT1 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human and mouse samples.

The MA5-15544 immunogen is purified recombinant fragment of WNT1 expressed in E. Coli.

MA5-15544 detects WNT1 which has a predicted molecular weight of approximately 41kDa.

Target Information

Wnt proteins constitute a family of secreted glyocoproteins that activate signal transduction pathways to mediate tissue homeostasis, cell fate, cell proliferation, and self-renewal. The canonical signaling pathway is initiated upon Wnt binding to a Frizzled family receptor and an LRP5/LRP6 coreceptor, which leads to activation of the beta-catenin cascade. Non-canonical Wnt signals are activated by binding to a Frizzled family receptor and ROR2 coreceptor. Extensive research has shown that deregulation of these pathways or its components can lead to cancer. Human Wnt-1 is encoded as a 370 amino acid precursor that, upon secretion, is cleaved into a 343 amino acid (42 kDa) mature form. At the amino acid level, mature human Wnt-1 is approximately 99% identical to mature mouse Wnt-1. Depending on the tissue, Wnt-1 can activate the canonical and non-canonical signaling pathways. Studies have demonstrated its involvement in a variety of functions, including embryonic development and carcinogenesis. Wnt-1 signaling can also induce the phosphorylation and subsequent degradation of c-Myb.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Protein Aliases: proto-oncogene Int-1; proto-oncogene Int-1 homolog; proto-oncogene protein Wnt-1; unnamed protein product; wingless-related MMTV integration site 1; wingless-type MMTV integration site family member 1; wingless-type MMTV integration site family, member 1 (oncogene INT1)

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Gene Aliases: BMND16; Int-1; INT1; OI15; sw; swaying; Wnt-1

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UniProt ID: (Human) P04628, (Mouse) P04426

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Entrez Gene ID: (Human) 7471, (Mouse) 22408

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Function(s)
receptor binding frizzled binding cytokine activity morphogen activity protein domain specific binding receptor agonist activity protein binding
Process(es)
embryonic axis specification cell-cell signaling positive regulation of cell proliferation response to wounding positive regulation of lamellipodium assembly negative regulation of cell-substrate adhesion Wnt signaling pathway cerebellum development central nervous system morphogenesis cerebellum formation neurogenesis negative regulation of cell-cell adhesion neuron differentiation negative regulation of BMP signaling pathway midbrain development cell proliferation in midbrain astrocyte-dopaminergic neuron signaling signal transduction in response to DNA damage negative regulation of apoptotic process positive regulation of insulin-like growth factor receptor signaling pathway cell fate commitment negative regulation of cell differentiation negative regulation of fat cell differentiation positive regulation of Notch signaling pathway positive regulation of transcription, DNA-templated positive regulation of fibroblast proliferation Spemann organizer formation canonical Wnt signaling pathway bone development positive regulation of dermatome development dopaminergic neuron differentiation negative regulation of oxidative stress-induced neuron intrinsic apoptotic signaling pathway midbrain dopaminergic neuron differentiation embryonic brain development negative regulation of cellular senescence branching involved in ureteric bud morphogenesis positive regulation of protein phosphorylation regulation of transcription, DNA-templated signal transduction myoblast fusion organ morphogenesis myotube differentiation spinal cord association neuron differentiation diencephalon development forebrain anterior/posterior pattern specification midbrain-hindbrain boundary maturation during brain development metencephalon development BMP signaling pathway negative regulation of transforming growth factor beta receptor signaling pathway midbrain-hindbrain boundary development organ regeneration T cell differentiation in thymus inner ear morphogenesis positive regulation of transcription from RNA polymerase II promoter neuron fate commitment neuron fate determination hepatocyte differentiation cellular response to peptide hormone stimulus hematopoietic stem cell proliferation negative regulation of protein ubiquitination involved in ubiquitin-dependent protein catabolic process
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