Search
Invitrogen
SKP1 Monoclonal Antibody (4E11)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
FIGURE: 1 / 9
SKP1 Antibody (MA5-15928) in ICC/IF
Immunofluorescence analysis of SKP1 was performed using 70% confluent log phase MCF-7 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with SKP1 Mouse Monoclonal Antibody (4E11) (Product # MA5-15928) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A2... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-15928
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate
Published species
Human,
Mouse
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
4E11
Immunogen
Purified recombinant fragment of human SKP1 expressed in E. Coli.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
Conc. Not Determined
Storage buffer
ascites
Contains
0.03% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-15928 targets SKP1 in indirect ELISA, FACS, IF, IHC, and WB applications and shows reactivity with Human samples.
The MA5-15928 immunogen is purified recombinant fragment of human SKP1 expressed in E. Coli. .
MA5-15928 detects SKP1 which has a predicted molecular weight of approximately 19kDa.
Target Information
This gene encodes a component of SCF complexes, which are composed of this protein, cullin 1, a ring-box protein, and one member of the F-box family of proteins. This protein binds directly to the F-box motif found in F-box proteins. SCF complexes are involved in the regulated ubiquitination of specific protein substrates, which targets them for degradation by the proteasome. Specific F-box proteins recognize different target protein(s), and many specific SCF substrates have been identified including regulators of cell cycle progression and development. Studies have also characterized the protein as an RNA polymerase II elongation factor. Alternative splicing of this gene results in two transcript variants. A related pseudogene has been identified on chromosome 7.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: cyclin A/CDK2-associated p19; cyclin-A/CDK2-associated protein p19; MGC34403; OCP-2; OCP-II protein; organ of Corti protein 2; organ of Corti protein II; p19skp1; polypeptide 1-like; RNA polymerase II elongation factor-like protein OCP2; S-phase kinase-associated protein 1A; SIII; transcription elongation factor B polypeptide 1-like; unnamed protein product
Gene Aliases: 15kDa; 2610043E24Rik; 2610206H23Rik; EMC19; OCP-II; OCP2; p19A; p19Skp1; SKP1A; TCEB1L
UniProt ID: (Human) P63208, (Mouse) Q9WTX5
Entrez Gene ID: (Human) 6500, (Mouse) 21402
protein binding
beta-catenin binding
protein domain specific binding
cullin family protein binding
molecular function activator activity
ubiquitin ligase complex scaffold activity
F-box domain binding
protein binding, bridging involved in substrate recognition for ubiquitination
ubiquitin ligase activator activity
ubiquitin-protein transferase activity
G1/S transition of mitotic cell cycle
protein polyubiquitination
chromatin remodeling
regulation of transcription, DNA-templated
ubiquitin-dependent protein catabolic process
cellular iron ion homeostasis
regulation of mitotic cell cycle
regulation of cell cycle process
regulation of centrosome duplication
neural crest cell differentiation
protein ubiquitination
regulation of BMP signaling pathway
SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
heterochromatin assembly
regulation of TOR signaling
regulation of circadian rhythm
regulation of apoptotic process
proteasome-mediated ubiquitin-dependent protein catabolic process
negative regulation of transcription, DNA-templated
regulation of inflammatory response
synaptic growth at neuromuscular junction
centrosome duplication
maintenance of protein location in nucleus
regulation of cell cycle
limb development
cilium morphogenesis
protein K48-linked ubiquitination
regulation of mitophagy
regulation of xenophagy
regulation of DNA damage checkpoint
protein monoubiquitination
apoptotic process
positive regulation of epithelial cell apoptotic process
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support