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Invitrogen
F4/80 Monoclonal Antibody (SP115)
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FIGURE: 1 / 16
F4/80 Antibody (MA5-16363) in ICC/IF
Immunofluorescence analysis of F4/80 was performed using 70% confluent log phase RAW 264.7 and NMuMG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with F4/80 Monoclonal Antibody (CI:A3-1) (Product # MA5-16363) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (P... View More
Product Details
MA5-16363
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Monoclonal
Type
Antibody
Clone
SP115
Immunogen
Synthetic peptide derived from N-terminus of mouse F4/80 protein.
Conjugate
Form
Liquid
Concentration
0.05 mg/mL
Amount
25 µg
Purification
Protein A
Storage buffer
PBS, pH 7.2, with 1% BSA
Contains
0.1% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
This antibody is predicted to react with rat based on sequence homology.
Heat-mediated antigen retrieval is recommended prior to staining, using an EDTA buffer for 10 minutes followed by cooling at room temperature for 20 min. A suggested positive control is human lung or mouse liver tissue.
Target Information
F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Adhesion G protein-coupled receptor E1; Cell surface glycoprotein F4/80; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; lymphocyte antigen 71
Gene Aliases: Adgre1; DD7A5-7; EGF-TM7; Emr1; F4/80; Gpf480; Ly71; TM7LN3
UniProt ID: (Mouse) Q61549
Entrez Gene ID: (Mouse) 13733
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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