Search
Invitrogen
CD4/CD8 Antibody Cocktail, FITC, PE
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
Product Details
MA5-16990
Product Specifications
Species Reactivity
Dog
Host/Isotype
Rat
/ IgG1/IgG2a
Class
Cocktail
Type
Antibody
Clone
YKIX302.9/YCATE55.9
Immunogen
Canine Concanavilin A activated T-cell blasts, Canine CD8 alpha chimaeric human IgG1 Fc fusion protein.
Conjugate
Form
Lyophilized
Concentration
Conc. Not Determined
Purification
purified
Storage buffer
PBS with 1% BSA, 5% sucrose
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-16990 contains a monoclonal mixture of FITC-conjugated CD4 (clone #YKIX 302.9, Rat IgG2a) and RPE-conjugated CD8 (clone #YCATE 55.9, Rat IgG1). This antibody is a dual-color reagent recognizing canine CD4 and CD8 cell surface antigens. Clone YKIX 302.9 recognizes canine CD4, which is expressed by lymphocyte subsets and canine neutrophils. Clone YCATE 55.9 recognizes CD8 alpha, which is expressed by cytotoxic T cells.
Reconstitute with 1 mL of distilled water. After reconstitution, store product undiluted at 4°C in the dark.
For FACS analysis, use 10 µL of the suggested working dilution to label 1x10^6 cells in 100 µL.
Target Information
CD4, a single chain transmembrane glycoprotein, is found on a T cell subset (helper/inducer) representing 45% of peripheral blood lymphocytes. It is also present on 80% of thymocytes and at a lower level on monocytes. It is involved in recognition of antigen presented along with MHC class II by APCs. It serves as receptor for HIV. The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a corepressor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains, or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: CD4; CD4 antigen (p55); CD4 antigen p55; CD8 antigen, alpha polypeptide (p32); CD8a; CD8alpha; CD8beta; Leu-2; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD8 alpha chain
Gene Aliases: CD4; CD8A
UniProt ID: (Dog) P33705, (Dog) P33706
Entrez Gene ID: (Dog) 403931, (Dog) 403157, (Dog) 483076
adaptive immune response
induction by virus of host cell-cell fusion
cell adhesion
T cell differentiation
macrophage differentiation
maintenance of protein location in cell
positive regulation of I-kappaB kinase/NF-kappaB signaling
T cell selection
positive regulation of monocyte differentiation
positive regulation of protein kinase activity
positive regulation of transcription, DNA-templated
positive regulation of viral entry into host cell
regulation of T cell activation
regulation of calcium ion transport
cellular response to granulocyte macrophage colony-stimulating factor stimulus
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support