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Invitrogen
MCP-1 Monoclonal Antibody (2D8)
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Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-17040
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Non-human primate
Published species
Human,
Mouse,
Rat
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
2D8
Immunogen
Purified recombinant fragment of human CCL2 expressed in E. Coli.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-17040 targets CCL2 in indirect ELISA, FACS, ICC, IHC, IF and WB applications and shows reactivity with Human, Mouse, and Non-human primate samples.
The MA5-17040 immunogen is purified recombinant fragment of human CCL2 expressed in E. Coli.
MA5-17040 detects CCL2 which has a predicted molecular weight of approximately 11kDa.
Target Information
This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: Ccl2; chemokine (C-C motif) ligand 2; chemotactic and activating factor precursor; Genbank Staff Annotation: The small inducible cytokine A2 (SWISS-PROT ACC P13500) is encoded in this mRNA, although it is interrupted and partial, perhaps indicating that this CDS is not the true product of the gamma.1 gene.; H-MCP-1; JE; MGC9434; monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; monocyte chemotactic and activating factor; monocyte chemotactic protein; monocyte chemotactic protein 1; monocyte secretory protein JE; Mutations: 171:OK; platelet-derived growth factor-inducible protein JE; RP23-350G1.3; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; small-inducible cytokine A2; unnamed protein product
Gene Aliases: GDCF-2; HC11; HSMCR30; JE; MCAF; MCP-1; MCP1; SCYA2; Sigje; SMC-CF
UniProt ID: (Human) P13500, (Mouse) P10148
Entrez Gene ID: (Human) 6347, (Mouse) 20296
angiogenesis
response to hypoxia
positive regulation of leukocyte mediated cytotoxicity
positive regulation of endothelial cell proliferation
leukocyte migration involved in inflammatory response
chronic inflammatory response
monocyte chemotaxis
positive regulation of leukocyte migration
positive regulation of cellular extravasation
cellular calcium ion homeostasis
inflammatory response
immune response
cytoskeleton organization
transforming growth factor beta receptor signaling pathway
glial cell migration
regulation of cell shape
response to xenobiotic stimulus
response to wounding
response to mechanical stimulus
response to bacterium
response to gamma radiation
regulation of vascular endothelial growth factor production
positive regulation of gene expression
positive regulation of macrophage chemotaxis
response to activity
negative regulation of angiogenesis
cytokine-mediated signaling pathway
sensory perception of pain
positive regulation of cell-cell adhesion
positive regulation of cell migration
neutrophil chemotaxis
organ regeneration
response to lipopolysaccharide
response to progesterone
positive regulation of tumor necrosis factor production
cellular response to insulin stimulus
positive regulation of collagen biosynthetic process
response to vitamin B3
negative regulation of glial cell apoptotic process
response to tumor necrosis factor
helper T cell extravasation
monocyte homeostasis
response to isolation stress
cellular response to macrophage colony-stimulating factor stimulus
cellular response to platelet-derived growth factor stimulus
chemokine (C-C motif) ligand 2 signaling pathway
response to amino acid
negative regulation of neuron apoptotic process
astrocyte cell migration
response to ethanol
positive regulation of cell adhesion
vascular endothelial growth factor receptor signaling pathway
eosinophil chemotaxis
macrophage chemotaxis
lymphocyte chemotaxis
positive regulation of synaptic transmission
positive regulation of T cell activation
positive chemotaxis
response to glucocorticoid
positive regulation of synaptic transmission, glutamatergic
mammary gland involution
maternal process involved in female pregnancy
maternal process involved in parturition
antimicrobial humoral immune response mediated by antimicrobial peptide
chemokine-mediated signaling pathway
cellular response to lipopolysaccharide
cellular response to retinoic acid
cellular response to ATP
cellular response to glucose stimulus
cellular response to interferon-gamma
cellular response to interleukin-1
cellular response to interleukin-6
cellular response to tumor necrosis factor
cellular response to estradiol stimulus
cellular response to fatty acid
cellular response to lipoprotein particle stimulus
cellular response to high density lipoprotein particle stimulus
cellular response to xenobiotic stimulus
cellular response to dexamethasone stimulus
positive regulation of monocyte chemotaxis
positive regulation of immune complex clearance by monocytes and macrophages
positive regulation of calcium ion import
positive regulation of wound healing
positive regulation of protein targeting to membrane
energy homeostasis
positive regulation of glutamate receptor signaling pathway
response to cyclosporin A
negative regulation of vascular endothelial cell proliferation
negative regulation of G1/S transition of mitotic cell cycle
positive regulation of endothelial cell apoptotic process
positive regulation of apoptotic cell clearance
negative regulation of natural killer cell chemotaxis
protein phosphorylation
chemotaxis
humoral immune response
cell adhesion
signal transduction
cell surface receptor signaling pathway
G-protein coupled receptor signaling pathway
G-protein coupled receptor signaling pathway, coupled to cyclic nucleotide second messenger
positive regulation of cytosolic calcium ion concentration
JAK-STAT cascade
organ morphogenesis
viral genome replication
cellular homeostasis
cellular response to fibroblast growth factor stimulus
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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