Search
Invitrogen
NCK1 Monoclonal Antibody (5B7)
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}
{{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}
Additional Information:
{{banner.description}}
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
MA5-17132
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Non-human primate
Host/Isotype
Mouse
/ IgG1
Class
Monoclonal
Type
Antibody
Clone
5B7
Immunogen
Purified recombinant fragment of human NCK1 expressed in E. Coli.
Conjugate
Unconjugated
Unconjugated
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Amount
100 µg
Purification
Protein G
Storage buffer
PBS
Contains
0.05% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
MA5-17132 targets NCK1 in FACS, IHC, indirect ELISA, and WB applications and shows reactivity with Human and Non-human primate samples.
The MA5-17132 immunogen is purified recombinant fragment of human NCK1 expressed in E. Coli.
MA5-17132 detects NCK1 which has a predicted molecular weight of approximately 47kDa.
Target Information
NCK1 is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
References (0)
Have you cited this product in a publication?
Let us know so we can reference it here.
Bioinformatics
Protein Aliases: cytoplasmic protein NCK1; melanoma NCK protein; MGC12668; NCK adapter protein 1; NCK tyrosine kinase; non-catalytic region of tyrosine kinase; SH2/SH3 adapter protein NCK-alpha; SH2/SH3 adaptor protein NCK-alpha; unnamed protein product
Gene Aliases: NCK; nck-1; NCKalpha
UniProt ID: (Human) P16333
Entrez Gene ID: (Human) 4690
protein kinase inhibitor activity
receptor binding
protein binding
cytoskeletal adaptor activity
protein domain specific binding
receptor signaling complex scaffold activity
protein binding, bridging
receptor tyrosine kinase binding
signaling adaptor activity
cadherin binding
ephrin receptor binding
eukaryotic initiation factor eIF2 binding
protein sequestering activity
molecular condensate scaffold activity
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription from RNA polymerase II promoter
signal complex assembly
cell migration
positive regulation of actin filament polymerization
response to endoplasmic reticulum stress
regulation of translation initiation in response to endoplasmic reticulum stress
positive regulation of translation in response to endoplasmic reticulum stress
positive regulation of T cell proliferation
T cell activation
positive regulation of transcription from RNA polymerase II promoter
negative regulation of insulin receptor signaling pathway
ephrin receptor signaling pathway
negative regulation of T cell receptor signaling pathway
antiviral innate immune response
positive regulation of endoplasmic reticulum stress-induced intrinsic apoptotic signaling pathway
positive regulation of cap-dependent translational initiation
positive regulation of cap-independent translational initiation
negative regulation of PERK-mediated unfolded protein response
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
Learn more
We're here to help
Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.
Contact tech support